• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2017, Vol. 19 ›› Issue (5): 460-461.DOI: 10.3969/j.issn.1671-2587.2017.05.015

• 临床检验 • 上一篇    下一篇

临床感染嗜水气单胞菌的产酶性和耐药性分析*

扈会整, 李俊海, 张晔婷, 任伟娟, 文杏, 王婵   

  1. 712000 陕西省核工业二一五医院
  • 收稿日期:2017-03-15 出版日期:2017-10-20 发布日期:2017-10-26
  • 作者简介:扈会整（1977-）,女,副主任检验师,硕士,主要从事微生物的检验与研究工作,（Tel）15384509179（E-mail）gj176224042@163.com。
  • 基金资助:
    *本课题受陕西省科学技术研究发展计划项目（No.2014K11-02-03-09）资助

Analysis of Enzyme Production and Drug Resistance of A.hydrophila

HU Hui-zheng, LI Jun-hai, ZHANG Ye-Ting, et al   

  1. No.215 Hospital of Xi'an Medical College,Xi'an
  • Received:2017-03-15 Online:2017-10-20 Published:2017-10-26

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摘要： 目的 了解嗜水气单胞菌的产酶现状和耐药性,为临床治疗嗜水气单胞菌的感染提供合理用药的依据。方法 收集2011~2015年,从临床感染标本中分离出嗜水气单胞菌50株。常规药敏试验采用K-B纸片法,超广谱β-内酰胺酶（ESBLs）依据2012年CLSI标准双纸片确证试验,头孢菌素酶（AMPC）应用三维试验改良法。结果 感染的50株嗜水气单胞菌,其中产ESBLs 15株（15/50）、产AMPC 10株（10/50）,且对氨苄西林全部耐药、对氨苄西林/舒巴坦钠耐药率为94%,对三、四代头孢菌素耐药率达40%,对其他药物保持较高的敏感性。结论 临床治疗嗜水气单胞菌引起感染时,应慎用头孢菌素类药物,根据药敏结果和耐药表型合理选用药物,为临床用药提供依据。

关键词: 嗜水气单胞菌, 药物敏感性试验, AmpC酶, 超广谱β, -内酰胺酶

Abstract: Objective To understand the current enzyme production and drug resistance of A.hydrophila forclinical treatment of A.hydrophila infection and to provide the basis for the rational use of antibiotics. Methods Fifty strains of A.hydrophilabacteria were collected from clinical specimens from 2011 to 2015.The routine drug sensitivity test was carried out using the K-B paper method,and the extended-spectrumβ-lactamases（ESBLs） was confirmed by the CLSI standard double disk tests.The AMPC was detected by three-dimensional test. Results Among 50 strains of A.hydrophila,15 strains produced ESBLs and 10 strains produced AMPC.All bacteria showed ampicillin resistance.Ampicillin/sulbactam resistant rate was 94%,and the third and the fourth generation cephalosporin resistant rate was 40% although the other antibiotics remained highly sensitive. Conclusion Clinical treatment of A.hydrophila infection should be careful with cephalosporins so as to improve the efficacy of antibiotics.

Key words: A.hydrophila, Antimicrobial sensitivity test, AmpC enzyme, Extended-spectrum β-lactamases

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