Abstract: ObjectiveTo investigate the expression and significance of inhibition of cell differentiation factor -1 (inhibitor of differentiation or inhibitor of DNA binding, id-1), vascular endothelial growth factor (vascular endothelial growth factor, VEGF) and matrix metalloproteinase-9 (matrix metalloproteinase-9, MMP-9) in normal cervical tissue, cervical carcinoma in situ and cervical squamous cell carcinoma. MethodsThe expression levels of Id-1, VEGF and MMP-9 proteins in normal tissues (n=20), cervical carcinoma in situ (n =16) and cervical squamous cell carcinoma(n =74)were examined by immunohistochemistry. ResultThe positive expression rates of Id-1, VEGF and MMP-9 in cervical squamous cell carcinora was gradually increased (P<0.05) than normal tissues, cervical carcinoma in situ, cervical carcinoma.The positive expression of Id-1, VEGF and MMP-9 was related to that of FIGO stage, histological stage(P<0.05), but it was not related to the age (P >0.05). Id-1 protein was positively related to that of VEGF (rs=0.809, P <0.05) and MMP-9 (rs=0.837, P <0.05). ConclusionThis study reveals that Id-1, VEGF and MMP-9 takes part in development and progression of cervical carcinoma. Both VEGF and MMP-9 ,and Id-1 are important biomarkers in the of diagnosis and prognosis of cervical carcinoma.

Key words: Cervical, carcinoma, Id-1, VEGF, MMP-9