The Influence of Platelets on Apoptosis of Lung Cancer Cells

doi:10.3969/j.issn.1671-2587.2019.01.007

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2019, Vol. 21 ›› Issue (1): 20-23.DOI: 10.3969/j.issn.1671-2587.2019.01.007

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The Influence of Platelets on Apoptosis of Lung Cancer Cells

LI Juan, LUO Yi-qing, FANG Man, et al

Departments of Laboratory Diagnostics and Blood Transfusion,the First Hospital of USTC,230001

Received:2018-08-11 Online:2019-02-20 Published:2019-01-28

Abstract

Abstract: Objective To study the impact of platelet on tumor cell apoptosis which is associated with resistance of lung cancer cells to chemotherapy. Methods Flow cytometry was performed to detect the apoptosis of A549 induced by PLT . The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP 2,MMP-9,the Bcl-2,Bax was examined by Western blotting after co-culture of PLT with A549 cells. Results The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP-2,MMP-9,and Bcl-2 was significantly upregulated while Bax was decreased after 48h cultrue. Conclusions Platelets facilitated migration of A549 cells in vitro,which was positively correlated with the platelet concentrations. Platelets increased the phosphorylation of ERK1/2,JNK,p-ERK,p-JNK; MMP-2 and the expression of MMP-2,MMP-9,and Bcl-2,but decreased the expression of Bax protein and inhibited the apoptosis of lung adenocarcinoma cells.

Key words: Platelets, Lung adenocarcinoma cancer cells, Apoptosis, Gemcitabine

CLC Number:

R331.1⁺43

LI Juan, LUO Yi-qing, FANG Man, et al. The Influence of Platelets on Apoptosis of Lung Cancer Cells[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(1): 20-23.

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The Influence of Platelets on Apoptosis of Lung Cancer Cells

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