Abstract: Objective Study on changes of related parameters in platelet from dogs with different concentrations of dimethyl sulfoxide in cryopreservation. Methods Platelets of experimental dog were prepared with 2% or 5% dimethyl sulfoxide concentrations and stored at -80℃ for cryopreservation from 1 to 180 days. After thawed, the stock of platelet were tested in platelet count (PLT), mean platelet volume (MPV), platelet count (PCT), platelet distribution width (PDW), platelet glycoprotein-Ib (GP-Ib), platelet selectin (P-Selectin), platelet factor- 4 (PF4), β-thromboglobulin (β-TG). Results The platelets after the cryopreservation with 2% or 5% dimethyl sulfoxide concentrations, which differences were observed in MPV, PDW (P<0.05), while the changes of PLT, PCT, GP-Ⅰb, P-Selectin, PF4, β-TG were not significant (P>0.05). With the prolongation of cryopreserved platelet, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4 and β-TG had different degrees of change in 1-180d except PLT (P<0.05). In addition, there was no significant differences between 2% and 5% dimethyl sulfoxide concentration for cryopreservation of platelets in PLT, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4, β-TG (P>0.05). Conclusion The 2 % and 5 % dimethyl sulfoxide concentrations could be chosen for deep cryopreservation of platelets in experimental dogs within 180 days, PLT, MPV, PCT, PDW, GP-Ib, P-Selectin, PF4, and β-TG was no significant difference between the two indicators. In order to reduce the residual amount of DMSO resulting in various effects of clinical animal tests, it is recommended that 2% dimethyl sulfoxide concentration be used.

Key words: Experimental dog, Platelets, Cryopreservation, Dimethyl sulfoxide