Abstract: Objective To study the molecular mechanisms of acute myelogenous leukemia stem cell apoptosis induced by triptolide. Methods Proliferation inhibition of triptolide to KG-1 cell was determined by MTT, IC50 concentration was calculated. According to the concentration gradient of triptolide, KG-1cells were collected to detect cell apoptosis via flow cytometry 48h after treatment. Bcl-2, beta-catenin, p-AKT and AKT proteins were detected by Western blot. Results Triptolide to KG-1 cell proliferation inhibition is in dose dependent manner and IC50 is about 4 nmmol/L, it can also induce apoptosis of KG-1 cell. Bcl-2, beta-catenin, p-AKT proteins can be down-regulated by triptolide. Conclusions Molecular mechanism of triptolide induced KG - 1 cell may be related to the Bcl-2, beta-catenin and p-AKT.

Key words: Triptolide, Myeloid leukemia stem cell, Apoptosis