In order to persistently ensure the safety and quality of blood products, pneumatic tube system of blood components transportation must be validated for its advantages and weaknesses before implementation for products delivery. The goal of this guideline is to provide direction for medical institutions planning to use pneumatic tube technology for blood component distribution. For the medical institutions that have already used pneumatic tube technology as a blood component delivery method, this guideline not only helps provide a comparison between the traditional methods and those of validated modern means, but establish guidelines to maintain a long-term quality assurance.
Objective To observe the influence of pneumatic tube system（PTS） on the partial functional parameters of red blood cells and platelets in blood. Method Thirty bags of whole blood in storage for 5 days was used, 450ml each. The blood was randomly divided into damping group and non-damping group, with 15 bags in each. Parameters of the blood cells before and after PTS transportation were measured by ELISA. Result The markers of 2,3-DPG, G6PD, sodium-potassium ATPase, CD55, CD59, GP-Ibβ, β-TG, PF4, and P-selectin were examined in damping group before and after transportation and compared with those in non-damping group. As a result, no significant difference was seen between the two groups (P>0.05). Conclusion PTS transportation for whole blood have not distinct and instant damage to red blood cells and platelets in stored whole blood.
Objective To observe the influence of pneumatic tube system (PTS) on some plasma coagulation factors and biochemical parameters. Method Thirty bags of whole blood were stored for 5 days in 450 ml each. Damping (for 15 bags) and non-damping (15 bags) methods of PTS transportation were used. The coagulation factors, lactic acid, and free hemoglobin were measured by ELISA, and plasma TP、Alb、LDH 、K+、Na+、CL- and Ca2+ were measured by automatic biochemical analyzer before and after PTS transportation. Results No significant difference was seen in the parameters of FI, FⅡ, FⅢ, FⅣ, FⅤ, FⅦ, FⅧ, FⅨ, FⅩ, FⅪ, FⅫ, FⅧ,TP, Alb, albumin/globulin ratio, lactic acid, LDH 、FHb 、K+、Na+、CL- andCa2+ before and after the PTS transportation no matter what methods were used (P>0.05). Conclusions Pneumatic tube system has limited impact on the plasma coagulation factors and biochemical parameters in whole blood. Immediate whole blood transfusion after transportation is safe in clinical blood transfusion.
Objective To study the influence of the store time and temperature in pneumatic tube system for whole blood transportation on ultrastructure of blood cells. Method Whole blood were stored for 5 days in 450 ml per bag and each was divided into 225 ml per bag. Damping and non-damping methods for PTS transportation were used. Blood cells were subjected to scanning electron and transmission electron microscopy to observe the cell ultrastructure in various store time and temperature. Result No instant striking difference of ultrastructure of the blood cells, such as RBCs, platelets and neutrophils, were noted between the two methods before PTS transportation. However, the time and temperature after 2 and 6 hr of blood storage following PTS transportation might notably affect the structure of the red cells, which has no close correlation with either damping or non-damping method, meanwhile, there was limited changes observed on platelets or neatrophils. Conclusion Pneumatic tube system has limited impact on ultrastructure of blood cells. Immediate whole blood transfusion after transportation by PTS is safe in clinical transfusion.
Objective To observe the Influence of pneumatic tube system on some parameters of whole blood cells. Method 30 bags of stored whole blood (450ml each) were selected to study, The bags of stored whole blood were randomly assigned to damping group or non-damping group, 15 bags in each group. Parameters of whole blood cells before the transportation were detected by Sysmex XS-800i automatic blood analyzer. The morphology of red blood cells stained with Methvlem blue and Eostm Y was observed by OLYMPUS microscope. Results There were no significant difference between damping group and non-damping group in parameters of whole blood cells (HB, HCT, RBC, MCV, MCH, MCHC, RDW, MPV, PCT, PLT, PDW, WBC) before and after the transportation(P>0.05). The statistical differences were only found in Hb, RBC, MCV, MCHC, PCT and PLT when the damping group and non-damping group stored for 6h after transportation (P<0.05). In addition, whether or not the damping measures are taken, the PTS has limited impact on the morphology of red blood cells in whole blood stored for 5 days when they were stored at a certain temperature and time after the transportation. Conclusions Pneumatic tube system has limited impact on parameters of whole blood. Immediate whole blood transfusion after transportation is safe in clinical blood transfusion.
Objective To study the impact of platelet on tumor cell apoptosis which is associated with resistance of lung cancer cells to chemotherapy. Methods Flow cytometry was performed to detect the apoptosis of A549 induced by PLT . The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP 2,MMP-9,the Bcl-2,Bax was examined by Western blotting after co-culture of PLT with A549 cells. Results The expression of ERK1/2,JNK,p-ERK,p-JNK,MMP-2,MMP-9,and Bcl-2 was significantly upregulated while Bax was decreased after 48h cultrue. Conclusions Platelets facilitated migration of A549 cells in vitro,which was positively correlated with the platelet concentrations. Platelets increased the phosphorylation of ERK1/2,JNK,p-ERK,p-JNK; MMP-2 and the expression of MMP-2,MMP-9,and Bcl-2,but decreased the expression of Bax protein and inhibited the apoptosis of lung adenocarcinoma cells.
Objective This study was aimed to compare the difference of qualities between two kinds Buffy-Coat-Collected Platelet Concentrates. Methods 400ml of whole blood were harvested from each of 124 healthy donors and then were separated and prepared to obtain the platelets through buffy coat method. 124 buffy coats were divided into 2 groups randomly, 53 buffy coats in experiment group were agitated overnight after standing 2 hours in platelet agitating container, while 71 buffy coats in control group were stewing overnight at room temperature. pH, GLU, HSR, platelet aggregation rate were observed in all platelet concentrates. Results There was no difference in platelet counts, pH and platelet aggregation rate between the two groups (P>0.05)．However, compared with the control group, platelets collected from agitated buffy coats had higher ability to hypotonic shock response and a higher rate of platelet recovery (P<0.05). Conclusion Two kinds of buffy-coat-collected platelet concentrates meet the requirement of relevant standards. Platelet concentrates from agitated buffy coats maybe have more advantages over those from stewing buffy coats in HSR and platelet recovery rate.
Objective To analyze the distribution characteristics of apheresis platelets donors in Tianjin from 2014 to 2016,and to provide evidence for further optimizing the strategies of recruiting and retaining unpaid blood donations to ensure the clinical blood supply. Methods The data of platelet apheresis blood donors were collected via SHINOW 9.0 modern blood management information system. Descriptive statistical analysis was carried out using SPSS 19.0 statistical software for the total number of blood donors and therapeutic doses,gender,blood type distribution,donation volume and the ratio of repeated blood donations. Results The number of apheresis platelets donors and the amount of therapeutic doses were increasing annually,a total of 83 411 donators gave their apheresis platelets in the past three years,and 125 098 treatment doses were collected. The majority of the blood donors were male,accounting for 84.81%; the blood types distribution included types B>O>A>AB; and 75.87% of the donors had their repeated donations. There were 68.69% of the donors in 2014,63.22% in 2015,and 68.04% in 2016 gave the blood volume equal to two therapeutic doses each time. Conclusion The apheresis platelets collection in Tianjin district showed a promising trend. A long-term strategy of the apheresis platelets donation recruitments should be put forward to ensure the safety and sufficiency of clinical apheresis platelets.
Objective To document the newly increased locations for blood donation and explore the effect of the locations for blood donation on the collection capacity. Method The data of the newly increased locations,experience of implementations,days of collection,and number and frequency of blood donations were summarized in Qinhuangdao area from 2015 to 2016. Results In one site where blood donation was facilitated,a total of 3 023 donations/times were completed,accounting for 19.27% of the whole collection capacity with 22.9 donators/times on average daily. Conclusions An example was set to improve the blood collection capacity and ensure the blood supply by optimizing the locations and appropriate organization in blood collection.
Objective To analyze the impact factors of perioperative nursing care in patients who underwent spinal surgeries. Methods Retrospective analysis was used to analyze the clinical data in 1 757 patients with transfusion on spinal surgery in 2017. Results A total of 1 525 cases in 1 757 transfusions on spinal surgery received suspended red blood cells, 1 401 cases were transfused with frozen plasma, 37 cases with cryoprecipitate, and 19 cases with apheresis platelets. The maximum volume was up to 6 000 ml and the minimum was 200 ml, with the average of 2 100 ml. Among them, eleven patients (0.71% ) had adverse transfusion reactions. Conclusion Blood transfusion helps the patients for early recovery after spinal surgery. Appropriate nursing may contribute to reduction of adverse reactions following transfusion therapy.
Objective To investigate the distribution of ABO and Rh blood groups in the Kirgiz population of Xinjiang region and explore the nationality-featured ABO and Rh phenotypes. Methods A total of 3 440 blood samples of ABO and Rh blood groups were identified in the patients. The value of expectation of ABO and Rh types, genome chimerism frequency and antigen frequency were calculated. The values of survey and expectations were compared for percent of contact area of Hardy-Weinberg. Results The distribution of ABO blood group was B>O>A>AB, and the frequency of AB blood type was the lowest (9.19%) while frequencies of A, B, and O types were 0.192 2, 0.240 7, and 0.567 1, respectively,which coincide with the law of genetic equilibrium. The negative rate of Rh type was 2.18%. No phenotype of CCdE was found but various Rh types were seen, including DCcEE（0.70%）, ccdee（0.78%）, ccdEe（0.23%）, Ccdee（0.12%）, CcdEe（0.03%）, and CCdee（0.03%）. Conclusion The Kirgiz nationality in Xinjiang has characteristic genetic frequency of Rh blood group,and has a different distribution of ABO blood group when compared with the Kirgiz population who have migrated to Heilongjiang, the northeast of China.
Objective To observe the change of erythrocytes and hemoglobin after fractural internal fixation surgery and analyze the effect of volume of liquid infusion on peripheral blood of patients during operation. Methods The levels of red blood cells (RBCs), hemoglobin, white blood cells and platelets at 24h, 48h or 72h following operation were detected and analyzed in 8 patients. Results The number of RBCs and concentration of hemoglobin were fluctuated on 24h, 48h, and 72h post-operation. The volume of infusion on operation had impact on the RBCs count and concentration of hemoglobin due to the blood dilution within 48h-72h, which increased significantly after 24h post-operation. Conclusions The volume of fluid infusion in orthopedic patients has a diluted effect on RBCs and hemoglobin but may gradually recover after operation.
Objective To observe the effect of plasma exchange （PE） on the treatment of patients with exertional heat stroke （EHS） underlying severe injuries of liver function. Methods Twelve male patients with EHS between the ages of 18 and 30 received plasma exchange therapy at early stage of the disease in addition to routine treatments from June 2013 to August 2016.The vital signs and health scoring（APACHEⅡ）of pre- and post-treatments were evaluated and ALT,AST,TBIL,CK,Cr,and D-Dimer were detected for prognosis judgement during the hospital stay. Results The life signs tended to be stable,the level of serum markers and APACHEⅡ scores decreased when compared with those before the novel therapy（P<0.05 or P<0.01）.Seven of 12 patients were recovered without any sequelae,three were improved and two died due to delayed transference to other hospital for further treatment. Conclusion The treatment strategy of plasma exchange may effectively improve the prognosis and reduce the mortality of EHS underlying severe hepatic lesions.
Objective To analyze the usage of fresh frozen plasma（FFP）in our hospital from April 2016 to March 2017,and to realize the appropriateness and effectiveness of FFP transfusion. Method Data werecollected through the hospital information system．The appropriateness and effectiveness of FFP transfusion was analyzed according to the evaluation criteria. Result Audit of FFP used shows appropriate utilization rate of 49.2% and effective rate of 33.9% in our hospital,No significant difference is found between surgical departments and non-surgical departments．There existed significant difference between the effectiveness rate of appropriate transfusion and inappropriate transfusion．The three largest reasons for inappropriate use were collocation of blood transfusion,active bleeding with INR<1.5 normal and the transfusion of FFP in the absence of bleeding. Conclusion Inappropriate usage of FFP is more and the effective rate of inappropriate transfusion is low．The hospital blood transfusion committees should be play an important role in monitoring the use of blood,training and supervising the medical staff,that helpfulimprove the appropriateness and effectiveness of FFP transfusion．
Objective To explore the clinical application value of the three parameter in the diagnosis and treatment of hepatitis B virus infection ,we analysed the relationship between pre S1 antigen (PreS1), hepatitis B virus nucleic acid (HBV-DNA) and hepatitis B virus serum markers (HBV-M). Methods A retrospective analysis was conducted on the detection data of PreS1, HBV-DNA and HBV-M in 1 184 patients with suspected HBV infection in our hospital from January 2016 to June 2018. Results There was a statistical difference between the PreS1 and the other six groups (χ2=87.597,111.736,216.866,168.015,421.362,324.532,202.062, P<0.05), and the positive rate of HBV-DNA in the "big three positive" group was significantly different from that of the other six groups (χ2=131.174,254.110,216.866,158.416,402.563,306.883. 181.859, P<0.05), the positive rate of PreS1 and HBV-DNA was much higher than that of other groups, and there was a significant association between PreS1 and HBeAg (χ2=478.981, P<0.001), and there was a significant correlation between PreS1 and HBV-DNA (χ2=909.450, P<0.001),the diagnostic coincidence rate between PreS1 and HBeAg was 85.13% and that between pre and HBV-DNA was 94.93%. the average viral load of HBV-DNA in patients with positive hepatitis B was higher than that in HBeAg negative hepatitis B patients (P<0.05). Conclusion the traditional serological detection of hepatitis B virus (HBV) HBeAg has certain correlation with PreS1 and HBV-DNA. PreS1 and HBV-DNA can more accurately reflect the replication status and contagion of HBV.
Objective To investigate the significance of total cholesterol and creatinine in prognosis of percutaneous coronary intervention （PCI） in patients with acute myocardial infarction （AMI） Methods 68 cases of PCI with AMI in the hospital were collected in 2016-2017. All subjects were divided into two groups of favourable （n=41） and unfavourable prognosis （n=27） according to the occurrence of adverse cardiac events in the follow-up period. The levels of total cholesterol and creatinine were compared before and after treatments. The ROC curve was used to evaluate the prognostic value of the treatments. Results The levels of total cholesterol and creatinine in patients with unfavourable prognosis were significantly elevated compared to those of unfavourable prognosis （P<0.05）. Pearson correlation analysis showed that the incidence of cardiac adverse events following PCI treatments was positively correlated with total cholesterol and creatinine （r=0.246,r=0.451,P<0.05）. ROC curve analysis showed that the detection sensitivity of total cholesterol combined with creatinine in AMI patients after PCI treatments was 81.48%,and the specificity was 85.37%. Conclusion The levels of cholesterol and creatinine are related to the severity of cardiovascular damage,and the combined measurements of total cholesterol and creatinine is useful for prediction of the prognosis of PCI patients with acute myocardial infarction.
Objective To evaluate the potential use of OSTA in bone density assessment in the elderly patients with chronic obstructive pulmonary disease （COPD）. Methods Based on risk stratification of OSTA,a correlation analysis of 120 patients with respiratory diseases were performed,among them 60 cases were COPD. Results No significant difference of osteoporosis at high-risk was seen when compared with the control. The OSTA was positively associated with bone density,weights and IC but negatively correlated to age,height,and concentrations of PINP and CTX,IL-8,IL-13,and TGF-β1. Conclusion The chronic obstructive pulmonary disease is a high risk factor of osteoporosis in senior patients and the OSTA is simple and economic for primary screening of osteoporosis in these patients.
Objective To evaluate the value of serum complement C3 and C4 in the diagnosis of autoimmune hepatitis patients. Methods From October 2011 to May 2017,87 patients with autoimmune hepatitis were treated with 44 cases of remission and 43 cases of active.Biochemical indicators of liver function in two groups by automatic biochemistry analyzer for detection of complement C3,C4,IgA,IgG,IgM,by turbidimetry was detected in peripheral blood,and detect liver biopsy. Results The levels of ALT,AST,TBIL,DBIL,GGT,ALP and IgG in patients with autoimmune hepatitis were significantly higher than those in patients with autoimmune hepatitis remission （P<0.05）. The level of autoimmune hepatitis （P<0.05）. The expression of C3 in all patients was significantly lower than that in patients with autoimmune hepatitis remission （P<0.05）. The expression of C3 in all patients was negatively correlated with AST,TBIL and DBIL （P<0.05）. There was no significant correlation between the expression of C3 and the levels of ALT,AST,TBIL,DBIL,GGT and ALP （P>0.05）. There was no significant correlation between ALT expression,ALT,ASTG,DBIL,GGT and ALP levels. The expression of C4 in 19 patients with autoimmune hepatitis was negatively correlated with （Histological activity index） HAI score （P<0.05）. Conclusion The levels of C3 and C4 in serum of patients with autoimmune hepatitis were significantly decreased,which provided a reliable basis for early diagnosis and early treatment.
Objective To explore the value of laboratory tests for the diagnosis of multiple myeloma. Methods The clinical data of bone marrow cell morphology, serum protein electrophoresis, immunoelectrophoresis and immunoglobulin in one patient with multiple myeloma were reviewed and analyzed. Results Immunoglobulin type conversion was seen in the patient following autologous hematopoietic stem cell transplantation. Conclusion The comprehensive laboratory tests may help to reduce the frequency of misdiagnosis of multiple myeloma. The changes of immunoglobulin type is of great significance for the clinical diagnosis and treatment of multiple myeloma.
Objective To investigate the effect of transportation temperature of the reagent on the quality and reliability of the results of enzymatic immunoassay. Method The purchased reagents were subjected to various temperatures simulated to the process of outside transportation. The quality of the reagents and reliability of tests were assessed. Results The quality of the reagents was remarkably decreased when they were exposed to 45℃±1℃ compared to their exposure to 37℃±1℃（P<0.05）. Conclusion Exposure of the reagents to high temperature during transportation may attenuate the quality of the reagents and results of laboratory tests,leading to missing of some weakly positive samples.
Objective we summarized early clinical characteristics of infants with Kawasaki disease,including clinical manifestations and laboratory examinations,in order to impove early-diagnosis. Methods Retrospective data from 21 cases of KD in infants was analysed,compared with 20 cases of KD in children older than 1 year in the same period. Results Compared with 20 cases of KD in children older than 1 year,the incidence of some clinical features,such as BCG vaccination erythrocyte,symptoms in digestive system and nervous system at early period were significantly（P<0.05）,while cervical lymphadenopathy was absent in 21 cases of KD in infants. The incidence of IKD cases in infants increased statistically（P<0.05）,Laboratory results,such as PLT,and the level of CRP,ALB,CK-MB have no difference except WBC and Hb. The cases of KD in infants have higher in WBC,but lower in Hb （P<0.05）.More cases of KD with hepatic injury or coronary artery aneurysm appear in infants（P<0.05）. Conclusion IKD cases and coronary artery aneurysm are more common in infants with KD.Not only the manual clinical features,but also BCG vaccination erythrocyte,symptoms in digestive system and nervous system and the level of WBC,Hb,PLT,CRP,ESR,ALT in time would be provide a clue for early diagnosis.Then completing the examination of coronary artery at the right time is necessary.
Objective To investigate the correlation between HCV RNA load and DARC rs12075 in HCV infected patients. Methods In 196 Dalian Han HCV infected patients,DARC rs12075 polymorphisms were typed by using TaqMan probe Real-time PCR and HCV RNA viral load was measured by Roche COBAS AmpliPrep/COBAS TaqMan with its supporting reagents. The HCV RNA viral load data were presented with beanplots,and the differences were compared through the Mann-Whitney U test. Results DARC rs12075 was genotyped and,171 FY*A/FY*A and 25 FY*A/FY*B patients were identified. The difference of HCV RNA viral load between FY*A/FY*A and FY*A/FY*B patients was not significant. In addition,we divided the patients into four groups according to HCV RNA viral load,and compared the HCV RNA viral load between FY*A/FY*A and FY*A/FY*B patients in each group. But the differences were not statistically significant. Conclusion Our results showed that HCV RNA load in peripheral blood of HCV patients was not related to DARC rs12075 polymorphism.
Objective To explore the diagnostic value of soluble triggering receptor expressed on myeloid cells-1 （sTREM-1） on postoperative infections following fractures. Methods A total of one hundred and eighty-seven patients with fractures treated in our hospital were divided into infection group （thirty-four cases） and non-infection group （one hundred and fifty-three cases） according to the infection standard,5 mL of veinal blood was collected 1 day before operation,immediate postoperative and 24 h after operation,respectively. The value of ESR was detected by Westergren method,the levels of CRP were measured by immunoturbidimetry assay,the level of PCT was detected by chemiluminescence immunoassay,and the levels of sTREM-1 were measured by enzyme linked immunosorbent assay. The diagnostic value of ESR,CRP,PCT,sTREM-1 for postoperative infections following fractures was performed by receiver operating curve （ROC）. Results Compared to the 1 day before operation,the levels of ESR,CRP,PCT,sTREM-1 in the infection group and non-infection group constantly increasing,there was significant difference between different time points （P﹤0.05）. The levels of ESR,CRP,PCT,sTREM-1 in the infection group were markly higher than that in the non-infection group,the difference between groups was significantly （P﹤0.05）. On the 1 day before operation,the accuracy of ESR,CRP,PCT,sTREM-1 for postoperative infections all were lower （all AUC﹤0.7）. At the moment of immediate postoperative,the AUC of PCT （0.951） higher than ESR （0.830）,CRP （0.738） and sTREM-1 （0.840）. On the 24 h after operation,the AUC of sTREM-1 （0.920） higher than ESR （0.731）,CRP （0.822） and PCT （0.780）. Conclusion Serum PCT can be used as an index for the early diagnosis of postoperative infections following fractures,while sTREM-1 can be used as an index for disease severity and course of infections.
Objective To study the relationship between CD80/CD86 expression in B cells and immune disorders in patients with immune thrombocytopenia（ITP）. Methods 90 patients with ITP were enrolled in the study,the flow cytometry was used to detect the expression CD80+/CD86+in CD19+B cells,the IgG/IgM level in peripheral blood. All patients were treated with recombinant human thrombopoietin （rhTPO） and prednisone,the complete remission rate was recorded by followed up,Logistic analysis was used to test the risk factors of the prognosis of ITP the partial correlation analysis was used to test relationship between CD19+CD80+,CD19+CD86+ and IgG,IgM expression. Results The patients were followed up for 3-11 months with an average follow-up time of （7.95±2.74） months,the complete remission rate of ITP was 46.67%. The multivariate analysis showed that CD19+CD80+,CD19+CD86+,IgG and IgM level were the high risk factors of prognosis （P<0.05）.The CD19+CD80+ and IgG,IgM had linear distribution,the partial correlation analysis showed significant positive correlation（r=0.229,0.592;P=0.031,P<0.001）.The CD19+CD86+and IgG,IgM had linear distribution,the partial correlation analysis showed significant positive correlation（r=0.415,0.292,P<0.001,0.005）. Conclusion The CD80 and CD86 are closely related to immune disturbances,and they are the high risk factors for the prognosis of patients with ITP,the over expression of CD80 and CD86 in B cells may be the important mechanism for over activation of CD19+B cells and mediated immune dysfunction.
Objective To establish the reference range of thyroid hormone at different stages of pregnancy,we analyzed the serum levels of major thyroid markers in healthy women in Wuhu area during pregnancy. Methods A total of 379 women during the different pregnancy were selected to detect TSH,FT3 and FT4 by Chemiluminescence Immunoassay. Another 118 healthy non-pregnant women were selected as control group。Analysis of TSH,FT3,FT4 three indicators in the different stages. Results Serum TSH,FT3,FT4 in the early pregnancy of the reference range were 0.38~4.07 mIU/L,3.55~ 5.30 pmol/L,11.35~17.43 pmol/L. The reference range in the middle of pregnancy was 0.41~4.14 mIU/L,2.99~5.20 pmol/L,10.48~17.79 pmol/L. In the late pregnancy reference range were 0.54~4.54 mIU/L,2.88~4.86 pmol/L,9.27~16.47 pmol/L. Conclusion The main serological indexes of thyroid in healthy pregnant women in Wuhu area were significantly different at different gestational periods,and we should establish a reference range for different pregnancies.
Objective To analyze the clinical and laboratory characteristics of Leukemia with t（11;19）（q23;p13）. Methods Twenty-one patients with （11;19）（q23;p13） were reviewed including the data of conventional cytogenetic analysis,fluorescence in situ hybridization （FISH）,fusion genes,and immunophenotype. Results Sixteen of 21 patients carry t（11;19）（q23;p13.1）,showing 11q+ and 19p- derivatives and positive MLL/ELL fusion genes. Seven males and nine females were definitively diagnosed,among them,one shows M2 ; 3,M4; and 10,M5. The other 2 cases were CMML. Five of 21 patients were t（11;19）（q23;p13.3）,exhibiting 11q- and 19p+ derivatives and positive MLL/ENL fusion genes. One male and four females were identified,3 cases were classified as proB-ALL,1 as preB-ALL,and 1 as T-ALL. Conclusions The t（11;19）（q23;p13） abnormality is one of the rare and non-random chromosomal translocations. The t（11;19）（q23;p13.1） are mostly seen in the patients with acute myeloid leukemia （AML） in adults. Comparatively,the t（11;19）（q23;p13.3） frequently occurs in the infants and children with malignancies of lymphoid lineage.
Objective This article compares the Blood Donation Law with other relevant laws and regulations and analyzes the inconsistencies or conflicting contents among them. The differences were found with age regulation of voluntary blood donors, publicity and education of voluntary blood donation, donation volume and time of intervals, treatments of surplus plasma, emergency blood usage and blood supply, punishments due to illegal blood collection, transfusion-transmitted infectious diseases, and dereliction of duty, etc. Suggestions were given to the blood collection and supply regulation system in the country, including the definition of unqualified blood products, protection of the privacy of blood donors, transportation privilege of first-aid blood delivery vehicles, and the occupational exposure treatments of medical personnel engaged in blood collection and preparation so as to to promote the sustained and stable development of voluntary blood donation and meet the needs for the development for health care.
Objective To evaluate the on-spot inspection capabilities of clinical routine chemistry and coagulation tests,and hepatitis B and C immunoassays in municipal,county and private hospitals. Methods Specimens were collected from 2016 to 2017 and external quality assessments of Anhui Provincial Center for Clinical Laboratories were used to design sample examination panels,then on-spot supervision and inspection were carried out. The results were uploaded to the network platform,the allowable range of big data was used as the criterion to evaluate the testing capabilities of the hospitals in different projects according to the international evaluation scheme. Results In clinical routine chemistry tests,coagulation tests and hepatitis B and C immunoassays,the average PT scores in municipal hospitals were 95.63,99.63 and 92.50 and the passing rates were 100%,100% and 87.5%,respectively. The averages PT scores of county hospitals were 94.46,99.50 and 82.66 with the passing rates of 96.97%,100% and 68.75%,respectively. The average PT scores of private hospitals were 89.50,90.81 and 82.50 with the passing rates of 93.75%,93.75% and 56.25%,respectively. Conclusion The average PT score of the three clinical examinations was found to be more than 80 and the overall testing ability is qualified but higher in city hospitals than in county and private hospitals successively. Among the three types of hospitals,the testing capabilities of clinical routine chemistry analysis tests and hepatitis B and C immunoassays are in good state but coagulation tests need to be improved.
