Abstract: Objective To investigate the effect of transportation temperature of the reagent on the quality and reliability of the results of enzymatic immunoassay. Method The purchased reagents were subjected to various temperatures simulated to the process of outside transportation. The quality of the reagents and reliability of tests were assessed. Results The quality of the reagents was remarkably decreased when they were exposed to 45℃±1℃ compared to their exposure to 37℃±1℃（P<0.05）. Conclusion Exposure of the reagents to high temperature during transportation may attenuate the quality of the reagents and results of laboratory tests,leading to missing of some weakly positive samples.

Key words: Temperature, Enzyme immunoassay reagents