Abstract: Objective This study was aimed to compare the difference of qualities between two kinds Buffy-Coat-Collected Platelet Concentrates. Methods 400ml of whole blood were harvested from each of 124 healthy donors and then were separated and prepared to obtain the platelets through buffy coat method. 124 buffy coats were divided into 2 groups randomly, 53 buffy coats in experiment group were agitated overnight after standing 2 hours in platelet agitating container, while 71 buffy coats in control group were stewing overnight at room temperature. pH, GLU, HSR, platelet aggregation rate were observed in all platelet concentrates. Results There was no difference in platelet counts, pH and platelet aggregation rate between the two groups (P>0.05)．However, compared with the control group, platelets collected from agitated buffy coats had higher ability to hypotonic shock response and a higher rate of platelet recovery (P<0.05). Conclusion Two kinds of buffy-coat-collected platelet concentrates meet the requirement of relevant standards. Platelet concentrates from agitated buffy coats maybe have more advantages over those from stewing buffy coats in HSR and platelet recovery rate.

Key words: Buffy coat, Oscillation, Statics, Platelet concentrates