Abstract: Objective The HPA genotyping of platelet donors in xingtai area was performed,and the HPA genotype of the platelet donors was established. Method PCR-SSP Method was used to randomly select 150 platelet donors who carry out a total of 14 antigens of HPA-1-6 and HPA-15 . Result The gene frequencies were found to be 0.953 and 0.010 for HPA-1a and -1b：0.951 and 0.086 for HPA-2a and -2b：0.596 and 0.394 for HPA-3a and -3b：1.000 and 0.000 for HPA-4a and -4b：0.967 and 0.015 for HPA-5a and -5b：0.986 and 0.010 for HPA-6a and -6b：0.475 and 0.506 for HPA-15a and -15b. Conclusion The gene frequencies of HPA-1-6 and HPA-15 antigens in xingtai area of Hebei province coincide with the Hardy-Weinberg genetic law,which shows difference among nationalities and regions.This is the first establishment of HPA genotype database in xingtai.

Key words: Xingtai region, HPA genotyping, Blood platelet