Abstract: ObjectiveTo identify 3 RhD variants by polymerase chain reactions（PCRs）. MethodsRh blood grouping was carried out by standard serologic test. PCR-SSP was used to amplify the RHD-specific polymorphisms located in RHD promoter,exons,introns,and RHCE-specific polymorphisms. RHD zygosity was determined by PCR-RFLP. ResultsThe serologic results showed that the 3 samples carry D variants. PCR-SSP results indicated that these patients harbored RHD*D-CE（3-6）-D hybrid alleles,ie,RHD*DVI.3. RHDzygosity showed the patients with RHD+/RHD- heterozygote. RHCE genotyping indicated that the patients have RHCE*Ccee. The RH genotyping of these three patients were CDVI IIIe/cde. ConclusionGenotyping is an efficient method in determining the RhD variants.

Key words: Rh, blood, group, RHD, gene, Rh（D）, variant, Genotype