Abstract: Objective To order to provide reference for clinical rational use of antibacterial agents，we investigated the distribution and drug resistance of Psedomonas aeruginosain in our hospital. Methods A retrospective study by the software WHONET 5 .6 and SPSS16 .0 was conducted on 474 strains of P . aeruginosa isolated from the clinical specimens collected from inpatients 2014 and 2016.The BioMerieux VITEK‐2 compact system was used to identify bacterial and sensitivity test . Results Totally 2407 strains of gram-negative bacteria were detected from the clinical specimens，including 474 strains of P .aeruginosa with the detection rate of 19.7%. The common types of specimens were sputum（76.4%），secretions（7.6%）. P .aeruginosa causing hospital-acquired infections were found frequently in intensive care unit（21.5%）and the department of respiration medicine（18.3%）;During the past three years，the isolation rate of Pseudomonas aeruginosa was always in the second place，the drug resistance rates to Trimethoprim/sulfamethoxazole，amikacin，tobramycin were significantly increased（P<0.05）. Conclusions Hospital-acquired infections with P .aeruginosa distributed in the departments of multi-invasive operations，widely use of antibiotics and long periods of hospitalization .Respiratory tract infection was prevalent，during the past three years，drug resistance of Psedomonas aeruginosa without significant descending trend. Therefore，bacterial resistance surveillance should be enhanced in order to provide the reference for clinical rational application.

Key words: Pseudomonas aeruginosa, Analysis of drug resistance , Clinical characteristic