Abstract: Objective Analyze the distribution characteristics of weak positive results of irregular antibody screening tests to explore the significance in clinical transfusion practice. Methods 32 684 patients requiring transfusion in our hospital between July 2017 and June 2019 were selected and samples were screened for irregular antibodies using antiglobulin test. The positive samples were further tested for antibody identification using saline test and antiglobulin test tube method. The distribution characteristics and possible reasons of agglutination reaction≤1+ results were analyzed. Results Of the 71 cases with weak positive antibody screening results, 31 were male and 40 were female,but there was no statistically significant difference（P>0.05）. The weak positive rate of irregular antibodies was not related to age（P>0.05）. 43 cases had a blood transfusion, and 28 cases had no history of blood transfusion; 52 cases were specific alloantibodies; 10 cases could not determine antibody specificity; 9 cases were negative for panelcell-16 antibody identification. Conclusion More attention should be paid to weak positive irregular antibody screening results, and provide compatible blood products to avoid blood transfusion ineffectiveness and adverse events of hemolytic transfusion reactions.

Key words: Antibody screening, Weak positive, Antibody distribution, Safe blood transfusion