Abstract: Objective To explore the clinical application of capillary ultracentrifugation in predicting massive maternal transfusion syndrome, which is more convenient to screen massive maternal transfusion syndrome with inconsistent blood types. Methods We mixed EDTA-anticoagulant blood of healthy women (type O on childbearing age) with type A/B umbilical cord blood for 1 hour, to simulate maternal transfusion syndrome. Fetal cells were isolated by capillary ultracentrifugation and performed blood group antigens identification. Furthermore, we performed flow cytometry to quantitatively verify the percentage of fetal red blood cells. Results When the fetal bleeding volume reached 60 and 100 mL, the results of sensitivity, Yoden index, positive predictive value and negative predictive value of capillary ultracentrifugation were respectively 0.8, 0.75; 0.75, 0.7; 0.941, 0.937 5 and 0.826, 0.791. Conclusions Capillary ultracentrifugation technique can serve as effective method for massive maternal transfusion syndrome with inconsistent ABO blood group.

Key words: Massive maternal transfusion syndrome, ABO blood group incompatibility, Capillary ultracentrifugation