Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice

doi:10.3969/j.issn.1671-2587.2021.04.008

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2021, Vol. 23 ›› Issue (4): 446-449.DOI: 10.3969/j.issn.1671-2587.2021.04.008

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Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice

MAO Yan, LU Li, XIAO Hui, et al

Department of Nephrology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008,Hubei,china

Received:2019-12-18 Published:2021-08-18

Abstract

Abstract: Objective To investigate the role and mechanism of Adenosine phosphate activating protease （AMPK） signaling pathway in the development of acute kidney injury in mice. Method 48 SPF BALB/C male mice were randomly divided into two groups：control group（n=24） and experimental group（n=24）. The experimental group were treated with adefovir dipivoxil 40 mg/kg,once a week for 2 times,while the control group were treated with equal amount of isotonic saline. Serum urea nitrogen（BUN）and creatinine（SCr） were measured by automatic biochemical analyzer. The expression of AMPK and Keap-1 in renal tissue were detected by Western blotting,and histopathological scores were also obtained. Results After 2 weeks,4 weeks,and 6 weeks of treatment,serum BUN and SCr values in the experimental group were significantly higher than those in the control group（P<0.05）,the histopathological scores of the kidneys in the experimental group were significantly higher than those in the control group （P<0.05）,and the relative expression levels of AMPK and Keap-1 protein in the kidneys of the experimental group were significantly higher than those in the control group （P<0.05）. Conclusion Acute kidney injury in mice can activate the AMPK signaling pathway and induce the expression of AMPK and Keap-1 increase,which leads to the deterioration of renal tissue and renal function.

Key words: Mice, Acute kidney injury, Adenosine phosphate activating protease, Renal function

CLC Number:

R692

MAO Yan, LU Li, XIAO Hui, et al. Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(4): 446-449.

References

[1] 王玉浔,安雅臣,蒋艳茹,等.N-乙酰半胱氨酸对脓毒症性急性肾损伤TLR4信号通路的影响[J].中华医院感染学杂志,2019,29(4): 493-497.
[2] AMARA V R,SURAPANENI S K,TIKOO K.Metformin attenuates cardiovascular and renal injury in uninephrectomized rats on DOCA-salt: Involvement of AMPK and miRNAs in cardioprotection[J].Toxicol Appl Pharmacol,2019,362:95-104.
[3] FENG J X,LI H Y,ZHANG Y F,et al.Mammalian STE20-like kinase 1 deletion alleviates renal ischaemia-reperfusion injury via modulating mitophagy and the AMPK-YAP signalling pathway[J].Cell Physiol Biochem,2018,51(5):2359-2376.
[4] 许武军,谢娟娟,陈仙.H2S通过抑制TLR4/MyD88/PI3K信号通路减轻尿源性脓毒血症诱导的急性肾损伤[J].中国病理生理杂志,2019,35(2): 243-247.
[5] FENG L F,REN J L,LI Y F,et al.Resveratrol protects against isoproterenol induced myocardial infarction in rats through VEGF-B/AMPK/ENOS/NO signalling pathway[J].Free Radic Res,2019,53(1):82-93.
[6] KIM K H,LEE I S,PARK J Y,et al.Cucurbitacin B induces hypoglycemic effect in diabetic mice by regulation of AMP-activated protein kinase alpha and glucagon-like peptide-1 via bitter taste receptor signaling[J].Front Pharmacol,2018,9:1071.
[7] 黄希杰,杨陈,安宁,等.Nrf2-Keap1信号通路在急性肾损伤中的研究进展[J].临床肾脏病杂志,2019,19(5):366-371.
[8] 杨霜,王丽.TLR4及其信号通路参与肾纤维化机制研究进展[J].医学研究生学报,2019,32(2):216-220.
[9] KODIHA M,FLAMANT E,WANG Y M,et al.Defining the short-term effects of pharmacological 5'-AMP activated kinase modulators on mitochondrial polarization,morphology and heterogeneity[J].PeerJ,2018,6:e5469.
[10] LIAO J,LIU B,ZHONG W,et al.Protective effect of Lycium barbarum polysaccharides against high-fat diet-induced renal injury and lipid deposition in rat kidneys[J].J Biol Regul Homeost Agents,2019,33(1): 7-17.
[11] LIEBERTHAL W,TANG M Y,ABATE M,et al.AMPK-mediated activation of Akt protects renal tubular cells from stress-induced apoptosis in vitro and ameliorates ischemic AKI in vivo[J].Am J Physiol Renal Physiol,2019,317(7):F1-F11.
[12] 林梦婕,白培进,陈仕智,等.老年抗中性粒细胞胞质抗体相关性血管炎合并肾脏免疫复合物沉积的临床与病理特点分析[J].中国全科医学,2020,23(23):2908-2912.
[13] 陈方旭,米焱,王彩丽.TGF-β/Smad、p38MAPK及JNK/SAPK信号通路在糖尿病肾病发生发展中作用机制的研究进展[J].山东医药,2019,59(9): 102-105.
[14] LIN Y C,WU M S,LIN Y F,et al.Nifedipine modulates renal lipogenesis via the AMPK-SREBP transcriptional pathway[J].Int J Mol Sci,2019,20(7): 1570.
[15] LIU B H,TU Y,HE W M,et al.Hyperoside attenuates renal aging and injury induced by D-galactose via inhibiting AMPK-ULK1 signaling-mediated autophagy[J].Aging (Albany NY),2018,10(12): 4197-4212.
[16] MAJD S,POWER J H T,CHATAWAY T K,et al.A comparison of LKB1/AMPK/mTOR metabolic axis response to global ischaemia in brain,heart,liver and kidney in a rat model of cardiac arrest[J].BMC Cell Biol,2018,19(1): 7.
[17] TSOGBADRAKH B,RYU H,JU K D,et al.AICAR,an AMPK activator,protects against cisplatin-induced acute kidney injury through the JAK/STAT/SOCS pathway[J].Biochem Biophys Res Commun,2019,509(3):680-686.
[18] WANG S Y,WANG W J,LIU J Q,et al.Methionine restriction delays senescence and suppresses the senescence-associated secretory phenotype in the kidney through endogenous hydrogen sulfide[J].Cell Cycle,2019,18(14):1573-1587.
[19] WANG Z Q,BAO W W,ZOU X B,et al.Co-expression analysis reveals dysregulated miRNAs and miRNA-mRNA interactions in the development of contrast-induced acute kidney injury[J].PLoS One,2019,14(7): e0218574.
[20] ZHANG J Y,CHEN Y,LUO H Q,et al.Recent update on the pharmacological effects and mechanisms of dihydromyricetin[J].Front Pharmacol,2018,9:1204.
[21] ZHAO W,ZHANG L,CHEN R,et al.SIRT3 protects against acute kidney injury via AMPK/mTOR-regulated autophagy[J].Front Physiol,2018,9:1526.
[22] LIU C M,YANG H X,MA J Q,et al.Role of AMPK pathway in lead-induced endoplasmic Reticulum stress in kidney and in paeonol-induced protection in mice[J].Food Chem Toxicol,2018,122:87-94.
[23] LIU Y M,YU H,ZHANG X H,et al.The protective role of autophagy in nephrotoxicity induced by bismuth nanoparticles through AMPK/mTOR pathway[J].Nanotoxicology,2018,12(6):586-601.
[24] LIU Z W,LIU H,XIAO L,et al.STC-1 ameliorates renal injury in diabetic nephropathy by inhibiting the expression of BNIP3 through the AMPK/SIRT3 pathway[J].Lab Invest,2019,99(5):684-697.
[25] LUO Y,WU M Y,DENG B Q,et al.Inhibition of soluble epoxide hydrolase attenuates a high-fat diet-mediated renal injury by activating PAX2 and AMPK[J].Proc Natl Acad Sci USA,2019,116(11):5154-5159.

Role and Mechanism of AMPK Signaling Pathway in the Development of Acute Kidney Injury in Mice

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