Cytogenetic and Molecular Genetic Studies on Chronic Lymphocytic Leukemia with t（18;22）（q21;q11）： Three Cases

doi:10.3969/j.issn.1671-2587.2021.04.014

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2021, Vol. 23 ›› Issue (4): 473-478.DOI: 10.3969/j.issn.1671-2587.2021.04.014

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Cytogenetic and Molecular Genetic Studies on Chronic Lymphocytic Leukemia with t（18;22）（q21;q11）： Three Cases

XIANG Xin, LIU Heng-fang, ZENG Zhao, et al

Jiangsu Institute of Hematology,Suzhou 215006

Received:2021-04-01 Online:2021-08-20 Published:2021-08-18

Abstract

Abstract: Objective To analyze clinical characteristics and laboratory features of chronic lymphocytic leukemia （CLL） with t（18;22）（q21;q11）. Methods CLL cells with DSP30+IL2 stimulant were cultured for 72 hours. Chromosome preparation and karyotype analysis were performed by routine method and R-banding technique. Furthermore, BCL-2 rearrangement in 3 cases was confirmed by fluorescence in situ hybridization（FISH）. Meanwhile,we presented a whole genome sequencing（WGS） of case No.1. Patients were followed-up for chemotherapy efficacy and survival. Results Abnormal karyotype described as t（18;22）（q21;q11） were showed in all 3 patients. FISH analysis revealed BCL2 rearrangement. WGS confirmed a BCL-2 and IGL translocations in case No.1. Conclusion t（18;22） has different molecular structure from t（14;18）and the incidence of abnormality is low. It is more common in CLL. CLL with this abnormality may be refractory and easy to relapse.

Key words: t（18;22）, （q21;q11）, CLL, BCL-2, IGL

CLC Number:

R733.72

XIANG Xin, LIU Heng-fang, ZENG Zhao, et al. Cytogenetic and Molecular Genetic Studies on Chronic Lymphocytic Leukemia with t（18;22）（q21;q11）： Three Cases[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(4): 473-478.

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Cytogenetic and Molecular Genetic Studies on Chronic Lymphocytic Leukemia with t（18;22）（q21;q11）： Three Cases

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