Abstract: Objective To study the hemostatic and effects of wound healing using chitosan-gelatin composite dressing loaded with platelet rich plasma（PRP）（CMC/GMs/PRP dressing） in a rabbit model of liver hemorrhage. Methods Twenty healthy New Zealand rabbits were randomly divided into 4 groups with 5 rabbits in each group：group A：control group,group B：CMC/GMs/PRP group,group C：negative control group （ordinary gauze）,group D：positive control group（Muyisha gauze）. The rabbit liver hemorrhage model was established. The hemostasis time and the amount of bleeding were recorded. The main hematological indicators and histological evaluations were performed at the first 1,2,3,4 weeks after the operation. Results The hemostasis time and bleeding volume in the CMC/GMs/PRP group were less than those in other three control groups（P<0.05）. During wound healing process,changes in white blood cell counts in the CMC/GMs/PRP group were minimal and repair of liver injury and safety in the CMC/GMs/PRP group were both better than those in other groups. Conclusion The PRP chitosan-gelatin composite dressing is capable of hemostasis and promoting wound healing, and also is safety. It has further research value and application prospects.

Key words: Platelet-rich plasma, Chitosan, Gelatin, Hemostasis, Healing