Clinical blood transfusion and blood transfusion-related immunity are critical to the survival of the transplant. With the improvement of transplantation technology, liver transplantation, kidney transplantation, small intestine transplantation,lung transplantation uterus transplantation, hematopoietic stem cell transplantation have been realized, and xenogeneic organ transplantation is also actively exploring. Researchers have gradually gained a new understanding of transplantation-related blood transfusion. This paper expatiated on blood immunology related to organ transplantation and hematopoietic stem cell transplantation, perioperative blood transfusion during transplantation and xenotransplantation in the future, in order to provide new ideas for blood transfusion in transplantation technology.

Objective We analyzed the postoperative blood transfusion in patients undergoing uterus transplantation (UTx),and discussed transfusion strategy and evaluated the efficacy. We formulated the transfusion plan to ensure the blood transfusion safety. Methods We retrospectively reviewed the clinical data of 2 cases of patients with UTx performed in January 2016 and January 2018. We analyzed hemoglobin,coagulation disorders and clinical blood use in postoperative period. We repeated compatibility testing. Red cells were transfused with blood type compatibility and non-red cells blood products were transfused with the same blood type. We observed changes in blood routine,coagulation,blood biochemistry and other indicators of patients before and after transplantation,and evaluated the effectiveness of transfusion. Results Perioperative changes in blood routine and coagulation functions in 2 patients with UTx were obviously caused by various factors. Leukoreduced and irradiated packed red blood cells were given after surgery for supportive treatment. Combined with multidisciplinary clinical treatment,patients recovered and were discharged from hospital. Conclusion The cause that hemoglobin drop acutely in uterine transplantation patient should be taken into account in many factors. While improving blood transfusion related serological examination,the clinical diagnosis and treatment process of patients should be comprehensively considered. Transfusion strategy and clinical treatment plan should be given to ensure patient safety.

Objective This study was to define independent risk factors of perioperative blood transfusion in patient who need a lung transplant using laboratory findings,operation methods and bleeding complications to provide the basis for clinical prediction of blood use and blood products preparation. Methods The medical records of 260 lung transplant patients in our hospital from July 2017 to July 2020 were retrospectively reviewed. These patients were divided into non-transfusion group（n=46）and blood transfusion group（n=214） according to receipt of perioperative blood transfusion. The clinical data and laboratory examinations were compared between two groups. Clinical and laboratory variables,such as patients' basic information,surgical conditions,preoperative and postoperative laboratory examinations,and extracorporeal membrane oxygenation（ECMO） related information,were compared using univariate analysis,and binary logistic regression were performed. Results In the univariate analysis,the difference between the two groups in disease course more than 7 years（P<0.05）,ASA Ⅳ（P<0.01）,double lung transplantation（P<0.001）,donor cold ischemia time>340 min（P<0.05）,lung transplantation duration>250 min（P<0.001）,blood loss>400 mL（P<0.001）,preoperative Hb reduction（P<0.05）,preoperative Hct reduction（P<0.05）,preoperative PT>15 s（P<0.05）,postoperative PT>15 s（P<0.05）,postoperative PTA<80%（P<0.05）,postoperative APTT>43.5 s（P<0.001）showed statistically significant.The index of P<0.1 in univariate analysis were performed in binary logistic regression analysis. The results showed that the postoperative APTT>43.5 s, double lung transplantation,intraoperative blood loss>400 mL,ECMO use,disease course>7 years were independent risk factors for perioperative blood transfusion in lung transplantation. Conclusion The postoperative APTT>43.5 s, double lung transplantation,intraoperative blood loss>400 mL, perioperative use of ECMO,and disease duration more than 7 years increased risk for blood transfusion. Evaluation of surgical methods and improvement of preoperative coagulation function and hemoglobin level are conducive to prepare blood products.

Objective To screen the population of rapid adaptation to high altitude hypoxia and the genes related to hypoxia adaptation by different population,including Han population who rapidly entered plateau (for 3 and 7day), the plateau-acclimatized Han population (residing for 30 and 90 days),the plateau Han population (more than 10 years on the plateau) and the Tibetan population. Methods The Blood routine examination and S_pO₂ data were measured by automatic blood cell analyzer and oxygen saturation detector. The mRNA expression levels of hypoxia inducible factor related genes were detected by qRT-PCR. The difference of HbF with high oxygen affinity was detected by the protein electrophoresis method. Results After entering the plateau the hemoglobin（Hb） concentration of Han population increased significantly（146.12±15.5 g/L in the plain,173.05±11.7 g/L after entering plateau 3 days,173.57±11.2 g/L after entering plateau 7 days,175.86±10.4 g/L after entering plateau 30 days,181.98±16.6 g/L after entering plateau 90 days, and 195.46±22.4 g/L living in plateau for a long time,P<0.05）,while S_pO₂ decreased significantly（97.28±1.46% in the plain,87.6±3.1% after entering plateau 3 days,and 90.4±2.6% after entering plateau 30 days,P<0.05）. The Hb concentration of Tibetan living in plateau was slightly higher than that Han population in plain,and S_pO₂ was slightly lower （P<0.05）. The expression level of EPAS1 mRNA was increasing in Han population after entering plateau for 7 days, 90 days,and living in plateau for a long time and in Tibetan（P<0.05）. The changes in RBC,Hb and Hct after entering the plateau 3 days were higher in Han population on the plains who did not have elevated EPAS1 gene expression than in those with elevated gene expression（P<0.05）. The HbF in Tibetan was 54.5% higher than that of Han population （more than 10 years on the plateau）with 27.3% and that of Han population living on plain with only 14.3%. Conclusion We should screen out the Han population,who were characterized with high expression of EPAS1 mRNA after entering plateau 3 days and high HbF,which provides a theoretical basis for the screening of Han population for rapid adaptation to hypoxia in the plateau.

Objective To investigate the role of DNA methylation of EPAS1 gene in the adaptation to hypoxia at high altitude by comparing the difference of DNA methylation levels in the EPAS1 gene promoter region among Tibetan group,plain Han group and plateau Han group （>10 years）. Methods Blood samples were collected from 30 healthy male volunteers of Tibetan, plain Han and plateau Han,and genomic DNA was extracted. Agena Massarray nucleic acid mass spectrometry was used to quantitatively detect the difference in the degree of DNA methylation among these groups. Total RNA was extracted from the above groups,and the reverse transcription was cDNA. The mRNA expression levels of EPAS1 in different groups were detected by qRT-PCR. Results The overall DNA methylation degree of EPAS1 gene promoter region was 8.87%±10.31%,7.17%±10.12%,9.32%±13.07% in the plain Han,plateau Han and Tibetan,respectively,and there was no statistical difference among the three groups（P>0.05）. The methylation levels of part of CpG sites in EPAS1 gene promoter region were significantly different among the three groups（P<0.05）. At the same time,the relationship between sequence mutations at rs13419896,rs1868092 and rs4953354 of EPAS1 gene and DNA methylation was analyzed. There was no statistical difference among the groups（P>0.05）. The expression level of EPAS1 mRNA was significantly higher in plateau Han than in plain Han,and slightly higher in Tibetan than in plain Han. Conclusion The DNA methylation level of some CpG sites in EPAS1 gene promoter region is different between Tibetan and Han population,and plateau Han may participate in the adaptation process of hypoxia through the change of DNA methylation.

Objective To establish prediction model of acute mountain sickness (AMS) based on radial basis function (RBF) neural network. Methods The hemoglobin, P₅₀, body mass index (BMI), single nucleotide polymorphism (SNP) of the EPAS1 and EGLN1 genes were detected in 98 people who urgent need to enter the plateau. According to the diagnostic criteria, AMS was judged after rapid ascent to high altitude. The diagnostic prediction model was established using RBF neural network. Results The correct percentage of the neural network learning training was 88.0%. The constructed neural network was used for prediction, and the coincidence rate between the results and the actual diagnosis results was 88.9%. The diagnostic ability of the prediction model was tested, and the area under the receiver operating characteristic (ROC) curve was 0.917, suggesting a good diagnostic ability. Conclusion The prediction model based on RBF neural network can provide an effective method for early diagnosis of AMS.

Objective To analyze the incidence,clinical features,and risk factors for death of neonatal alloimmune thrombocytopenia（FNAIT）in Chinese population,in order to summarize the epidemiological characteristics of FNAIT in Chinese population and provide support for the development of prevention and control strategies. Methods We retrieved from Medline,PubMed,CBM,CNKI,Wanfang Database,VIP Database and Baidu Academic Search Engine for case reports of FNAIT published from January 1985 to January 2021. The literature was screened according to the inclusion and exclusion criteria,and the literature data was retrospectively analyzed using SPSS 21.0 software.. Results A total of 21 studies were included,involving 36 children. 27 children had positive platelet antibodies,and 9 cases were diagnosed by clinical symptoms and platelet count after exclusion of other thrombocytopenia diseases. Among the children,20 cases were males and 14 cases were females（2 cases were missing）,and the median age of onset was 1 day. There were single fetus in 33 cases and twins in 3 cases. The platelet count of mothers was normal with 17 primiparas and 19 multiparas. The median platelet count in the children was 27.5×10⁹/L,and the clinical manifestations were petechiae,gastrointestinal bleeding and intracranial hemorrhage. After immunoglobulin,glucocorticoid therapy and blood transfusion,2 cases died or died intrauterine（5.9%）. Logistic regression analysis showed that maternal pregnancy and birth history,fetal platelet count, fetal gastrointestinal bleeding and intracranial hemorrhage did not be the risk factors affecting the prognosis of children. Conclusion In China,the incidence of FNAIT is low,and the prognosis of neonatal patients is relatively high after conventional treatment. Routine platelet antibody screening for pregnant women is not recommended.

Objective To compare the characteristics of blood indexes of different diseases by analyzing the disease spectrum of hospitalized blood transfusion newborns in China and the blood test indexes of the top five diseases before admission without intervention treatment, so as to provide data support for accurate treatment of newborns. Methods A multicenter retrospective survey method was used to collect the relevant diagnosis and treatment data of 5 669 hospitalized newborns who received blood transfusion treatment in 46 tertiary public hospitals in China from January 1, 2017 to June 30, 2018. Statistical analysis was carried out according to the main disease diagnosis of the International Classification of Diseases Code (ICD-10) to obtain the disease spectrum of hospitalized newborns with blood transfusion. The routine blood test indexes, liver function indexes, blood coagulation function and blood gas indexes of the newborns after admission before treatment and intervention were analyzed to obtain the range of blood test indexes for different diseases before intervention. Results The first five diseases were premature infants (25.44%), neonatal pneumonia (15.82%), neonatal hyperbilirubinemia (10.65%), neonatal hemolytic disease (6.40%), and neonatal respiratory distress syndrome (6.07%). The routine blood test indexes, liver function indexes, coagulation function and blood gas indexes of the first five diseases were statistically significant (P＜0.05). The routine blood test indexes (RBC, HGB, HCT, WBC), coagulation indexes (PT, APTT, INR), liver function indexes (ALT, TBIL, TP, ALB), blood gas (PO₂ and SO₂) of neonatal pneumonia and neonatal respiratory distress syndrome were statistically significant. The routine blood test (RBC, HGB, HCT, WBC), coagulation function (APTT, FIB), liver function (TBIL, TP), blood gas (PH, PO₂, HCT) of neonatal hyperbilirubinemia and neonatal hemolytic disease were statistically significant. Conclusion Premature infants, respiratory diseases and hyperbilirubinemia are the main diseases of neonatal blood transfusion in China, and there are differences in blood test index values of different diseases, suggesting that accurate individual diagnosis and treatment should be carried out according to different diseases for neonatal diagnosis and treatment.

Objective To explore optimizing transfusion ratios of different blood products in postpartum hemorrhage requiring massive blood transfusion (MT). Methods A retrospective study was performed using data from all patients who underwent a MT in the Obstetrics Department of the Third Affiliated Hospital of Guangzhou Medical University from May 2018 to April 2021. The information including patient's general condition, cause of postpartum hemorrhage, amount of blood loss, amount of blood products and patient outcomes were collected. Results Placental factors was the main cause of postpartum hemorrhage (84.00%), of which 69.00% was caused by placenta accreta. One hundred patients with postpartum hemorrhage received a MT, and the bleeding of 84 patients were well controlled. The mean estimated blood loss was 2 778 mL. Each patient required blood transfusion at a mean of 8.6 units of packed red blood cells, 543 mL of fresh frozen plasma and 352 mL of frozen plasma. Among the five groups of patients with postpartum hemorrhage who received MT, group 1 (red blood cell∶plasma=1∶1) and group 3 (red blood cell∶plasma=6∶4) had the highest blood transfusion efficiency (96.88%, 94.12%). Conclusion A reasonable mass transfusion program is an important treatment for postpartum hemorrhage. A 1∶1 program of red blood cells and plasma is recommended, then 6∶4 program of platelets and/or cryoprecipitate should be given as early as possible.

Objective To investigate the effect of Lymphoplasmapheresis (LPE) on patients of ABO-incompatible living donor kidney transplantation (ABOi-KT). Methods Eight ABOi-KT patients received LPE treatment 57 times before surgery. Blood cell count and plasma protein content were compared before and after LPE treatment. Perioperative blood group antibody titers were monitored. The occurrence of adverse reactions was analyzed. Results There were no significant differences in white blood cell count, neutrophil count, hemoglobin and red blood cell count between before and after LPE treatment (P>0.05). Lymphocyte count and platelet count decreased after treatment (P<0.05). There were no significant changes in plasma total protein and albumin between before and after treatment (P<0.05). The amount of globulin decreased after treatment (P<0.05). Each patient received several LPE treatments to ensure that the blood group antibody titers on the day of ABOi-KT surgery was less than 1∶16, and there was no titer rebound in two weeks after surgery. The incidence of adverse reactions in patients received LPE treatment was 8.8%. Conclusion Preoperative LPE treatment for ABOi-KT patients is helpful for safe and effective operation.

Objective To study the hemostatic and effects of wound healing using chitosan-gelatin composite dressing loaded with platelet rich plasma（PRP）（CMC/GMs/PRP dressing） in a rabbit model of liver hemorrhage. Methods Twenty healthy New Zealand rabbits were randomly divided into 4 groups with 5 rabbits in each group：group A：control group,group B：CMC/GMs/PRP group,group C：negative control group （ordinary gauze）,group D：positive control group（Muyisha gauze）. The rabbit liver hemorrhage model was established. The hemostasis time and the amount of bleeding were recorded. The main hematological indicators and histological evaluations were performed at the first 1,2,3,4 weeks after the operation. Results The hemostasis time and bleeding volume in the CMC/GMs/PRP group were less than those in other three control groups（P<0.05）. During wound healing process,changes in white blood cell counts in the CMC/GMs/PRP group were minimal and repair of liver injury and safety in the CMC/GMs/PRP group were both better than those in other groups. Conclusion The PRP chitosan-gelatin composite dressing is capable of hemostasis and promoting wound healing, and also is safety. It has further research value and application prospects.

Objective To analysis the common panel cells for antibody identification in the domestic market, to study the antigen pattern, antigen coverage, and matters needing attention in analyzing and judging the identification results. Methods 20 batches of domestic panel cells (G1~G20) and 11 batches of imported panel cells (J1~J11) were collected for quality evaluation from the aspects of antigen composition pattern, antigen coverage rate, antigen negative and positive ratio and homozygote or not. The reliability of antigen was analyzed by Fisher's exact test. Results 20 batches of domestic cells were covered with 29 antigens, the coverage rate was 76.00%±34.3%, and 11 batches of imported cells were covered with 28 antigens, the coverage rate was 83.00%±21.9%. Compared the two groups of panel cells, the imported panel cells lack antigens: Mur、Di^a、Di^b、Do^a、Do^b、Yt^a、Yt^b; the domestic panel cells lack antigens: C^w、V、f、Js^a、Js^b、Xg^a; the unreliable antigen ratio of 20 batches of domestic panel cells in identifying irregular antibodies is 49.16%±7.78%, and that of 11 batches of imported panel cells is 27.61%±5.48%.The proportion of homozygotes in imported cells was 73.15%±4.59%. Conclusion The antigen coverage of domestic panel cells is similar to that of imported panel cells, but the reliability of antibody identification is significantly lower than that of imported panel cells. It is necessary to increase the number of panel cells or change the pattern of antigen composition to improve the reliability of irregular antibody identification. The imported panel cells do not contain Di^a antigen, Mur antigen and other antigens with high frequency in Chinese Han population, so they are not suitable for Chinese Han population. Because blood group antigens have ethnic and regional polymorphism, and irregular antibody distribution also has ethnic and regional polymorphism, it is suggested to design panel cells suitable for screening irregular antibody according to the ethnic concentrated areas, so as to play a greater role in clinical safe and effective blood transfusion and prevention of hemolytic disease of fetus and newborn.

Objective We established a method of sequence analysis for human erythrocyte Rhesus D （RhD） blood group protein to lay a foundation for identifying the expression of RhD blood group gene in erythrocyte membrane. Methods Five mL of anticoagulant blood from 10 blood donors with RhD positive were randomly selected. The Rh blood group antigen protein was immunoprecipitated. Rh（Rhesus）protein was extracted from erythrocyte membrane by protease hydrolysis and quantified. After being cleaved by protease,the Rh protein sequences were identified by mass spectrometry （LC-MS）. Results The method for detecting RhD protein sequence was established and the standard curve of RhD protein was developed. The concentrations of RhD proteins in 10 samples were obtained. The RhD target protein and RhD protein fragment from Basic Local Alignment Search Tool（BLAST）platform exhibited 100% sequence identity,and a secondary mapping of RhD protein peptide in the erythrocytic membrane was obtained. Conclusions Immunoprecipitation coupled to mass spectrometry can define amino acid sequence of RhD protein in red blood cells to lay a solid foundation for verifying RhD antigens expressed on red blood cells. It can accurately determine RhD blood group and has a very wide application.

Objective To explore the application value of idebenone in platelet preservation through its effect of on platelet biological characteristics. Methods In vitro experiment, platelet-rich plasma was added idebenone with the final concentration of 10 μg/mL as the experimental group. We detected the platelet adhesion and aggregation function, plasma malondialdehyde (MDA) concentration, reduced glutathione (GSH) concentration, and CD62p expression on platelet membrane surface and JC-1 on mitochondrion surface. Results Compared with the control group, platelet adhesion and aggregation rate decreased to a certain extent（P<0.05）; The MDA level decreased（P<0.05）while reduced GSH level increased（P<0.05）; The positive rate of CD62p showed no significant change, but the positive rate of JC-1 increased significantly（P<0.05）. Conclusion Idebenone would be beneficial to the metabolism of platelet mitochondria, but it has a certain inhibitory effect on platelet adhesion and aggregation with the concentration of 10 μg/mL.

Objective To study the RhCE phenotype and RHD genotype of blood donors with weak D phenotype in Shenzhen. Methods Blood samples were collected from 38 donors. The D blood group of these samples was tested using anti-D monoclonal IgM/IgG blood identification reagent. The CE phenotype was identified using monoclonal anti-C, anti-c, anti-E, and anti-e. The 10 exons of RHD gene were analyzed by PCR-SSP. The full length coding region of RHD gene was sequenced if necessary. The correlation between RhCE phenotype and RHD genotype was analyzed. Results Serology results showed that the 38 donors were D variant. In 38 donors, eight RHD alleles have been reported, RHD*weak partial 15(15/38), RHD*DEL1(11/38), RHD*DVI.3(5/38), RHD*weak D type 61(1/38), RHD*weak D type 95(1/38), RHD*DCC(1/38), RHD*DFR1(1/38), RHD*weak D type 50(1/38), RHD*weak partial 15/RHD*DEL1(1/38) were identified. Additionally, RHD*weak D type 50 was not been reported in the Chinese Han individual. Also, one novel RHD*IVS9-1C(c.1228-1G>C) allele were identified. The nucleotide sequence data of the new RHD allele was submitted to GenBank, and was assigned accession number MT755965. The correlation coefficient between RHD*weak partial 15 and RhE antigen was 0.727 (P<0.01). The correlation coefficient between RHD*DEL1 and RhC antigen was 0.645 (P<0.01). Conclusion The molecular background of weak D in Shenzhen is complicated, which mainly includes RHD* Weak Partial 15, RHD*DEL1, RHD*DVI.3, and other rare genotypes. RHD* Weak Partial 15 was associated with RhE antigen and RHD*DEL1 was associated with RhC antigen.

Objective To study the methods for reentry of NAT reactive blood donors to reduce blood scrapping. Methods Blood donors with NAT reactivity and other blood screening non-reactive were selected between 2012 and 2017 in Shanghai Blood Center, and 42 blood donors were recalled to the reentry program. Serological detection methods (ELISA, CLIA) and NAT methods were used to detect the markers (HBV, HCV, HIV and TP). Methods for reentry of ELISA-/NAT+ blood donors were designed. Results In the first reentry, 15 cases were ELISA non-reactive and NAT reactive; 27 cases were ELISA non-reactive and NAT non-reactive. Among 27 cases, 9 cases were anti-HBc/anti-HBe reactive and 14 cases were anti-HBc reactive. Anti-HBc and / or anti-HBe reactive blood donors in the first reentry were still reactive in the second reentry, which suggested those were all deferred permanently in the first reentry. Four reentry cases that were non-reactive to all tests may be eligible for reentry. Conclusion We studied the feasibility of reentry of NAT reactive blood donors and preliminarily determined the reentry rule.

Objective To analyze the serological and molecular characteristics of ABO subgroups among blood donors in Wuxi. Methods A total of 19 samples from blood donors between May 2016 and June 2019 were preliminarily identified as ABO subgroup using serological testing. Fourteen samples were identified using molecular biology techniques, 11 of which were identified by both Polymerase Chain Reaction Sequence-specific Primer (PCR-SSP) and DNA sequencing and the other 3 samples were only identified by DNA sequencing. Results Among 120 118 blood donors, the frequency of ABO subgroup using serologic testing was approximately 1.6/10 000, and the number of B subgroup was more than that of A subgroup. Nine ABO mutant alleles, including Ael02, A201, Bw27, Bel03, Bw22, B310, A217, cisAB01, B(A)02 were found. Moreover, no mutation in 3 samples were charactered in exon 1-7 of the ABO gene, and a new allele was found (GenBank: MT281528). Conclusion A combination of serologic testing, PCR-SSP and DNA sequencing can more accurately identify ABO subgroups.

Objective To analyze the stability of hepatitis C virus（HCV）in serum and its purified nucleic acid at different temperatures. Methods Anti-HCV-negative plasma obtained from the Blood Center was used as the diluent. The 10⁷ IU/mL HCV serum was diluted to 10⁵ IU/mL and 10³ IU/mL. Total RNA was extracted and purified. Fluorescence quantitative polymerase chain reaction（FQ-PCR）was used to determine three serum concentrations. The remaining serum and its nucleic acid were packed into 45 aliquots and stored at 25 ℃, 4℃and -20 ℃ respectively. The serum and nucleic acid were taken out to detect viral load changes after storage at 1 day, 3 days, 6 days, 9 days, 12 days, 15 days, 18 days, 21 days, 28 days, 35 days, 42 days, 49 days, 56 days, 63 days and 70 days. The experiments were repeated three times, and the mean and standard deviation were calculated. Results HCV viral load showed a downward trend over extended storage time（P<0.05）; the viral loads were correlated to time, storage temperature and initial concentration（P<0.05）; there were significant differences between10⁷ IU/mL、10⁵ IU/mL and 10³ IU/mL storage for the same time and at different temperatures（P<0.05）. There was no significant difference in HCV viral load between at 4 ℃and -20 ℃（P>0.05）, but there was significant difference between at them and 25 ℃（P<0.05）. HCV purified nucleic acid concentration levels of 10³ IU/mL, 10⁵ IU/mL and 10⁷ IU/mL were stored at 25 ℃, 4℃ and -20 ℃ for 70 days, and the concentration change was negatively correlated with the initial concentration（P<0.05）. Conclusion HCV serum stored at 4 ℃or -20 ℃ can maintain the stability. The higher the viral load, the better the stability. HCV purified nucleic acid was stored at 25℃, 4 ℃and -20 ℃ for 70 days, and its concentration change was negatively correlated to the initial concentration.

Objective To provide a basis for the recruitment and management strategies of donors, we tested whether there were the biochemical changes of blood in donors who plateletpheresis 20 consecutive times and with an interval of 14~16 days, and evaluated the impact of shortening the donation interval. Methods The test results of blood samples taken from donors before plateletpheresis were used as subjects. A total of 42 male cases, aged 25~54, and with an interval of 14~16 days. They were divided into three groups: the test results of the blood samples taken before the first plateletpheresis were used as the control group, and the 10th and 20th test results were used as the observation group 1 and the observation group 2. Blood biochemical tests include: ALT, Alb, TP, Ca, P, Fe and SF. Results Compared with the control group, there was no significant difference in the ALT, Alb, TP, Ca, P and Fe detection results of blood donors between observation group 1 and group 2 (P>0.05). However, there was a statistically significant difference in the SF test results of blood donors among observation group 1 and group 2 and the control group (P<0.05). Conclusion There was no significant change in ALT, Alb, TP, Ca, P, and Fe in male donors who plateletpheresis 20 consecutive times and with an interval of 14~16 days, but the SF would decrease if more than consecutive 10 times.

Objective Study on the application of standard script in the communication of blood donors,provide reference for standardized, warm and accurate communication with donors. Methods According to the needs of blood collection and supply work, we should formulate reasonable communication objectives, timely update the knowledge base of FAQ, analyze the psychological characteristics of blood donors such as individual or group expectations, needs, motivation, etc., use effective psychological strategies, design standard scripts, and constantly summarize and improve in the application. Results The communication training cycle of new employees was shortened from 3 months to 1 month. From 2017 to 2019, a pheresis platelet donors successfully recruited 3 593, 4 131 and 4 440 respectively, and the difference was statistically significant compared with 2014~2016 (t=4.230,P<0.05), and the service satisfaction was increased to 99.2% in 2019, meanwhile zero complaint of communication service. Conclusion The application of standard scripts promotes personnel training, shortens the growth cycle, standardizes the communication service of donation, ensures the quality of communication, promotes the recruitment of donors,and improves the satisfaction of donors.