Abstract: Objective To identify three novel alleles of HLA-A, analyze their nucleotide and amino acid sequences. Methods Three novel alleles were initially detected by DNA sequencing typing (Sanger sequencing), then the sample sequenced by Next Generation Sequence (NGS) to confirm the mutation allele and locus. Results The first new allele sequence was different from that A*02:07:01 at the position 520 (G>A) in exon 3, which results in an amino acid change from Ala to Thr at codon 174. The second new allele sequence was different from that A* 02:06:01 at the position 641 (C>T) in exon 4, which results in an amino acid change from Thr to Ile at codon 214. The third new allele sequence was different from that A* 02:01:01 at the position 652 (G>C) in exon 4, which results in an amino acid change from Val to Leu at codon 218. Conclusion All three alleles were new HLA-A alleles which have been designated as: HLA-A*02:897, HLA-A*02:898, HLA-A*02:899 by the Word Health Organization (WHO) HLA Nomenclature Committee.

Key words: HLA allele, Amino acid change, Next Generation Sequence