Abstract: Objective To investigate the genetic polymorphism of HPA1-5 alleles in people of childbearing age from Yunnan Province and to provide valuable data for diagnosis and treatment of FNAIT and other diseases. Methods PCR-SSP was adopted to genotype the HPA1-5 system in 576 cases from different nationalities, Gene frequency and noncooperative rate of the population in Yunnan were calculated and were compared with each other. Platelet antibody screening was carried out among 630 women of childbearing age in Yunnan, andresults were analyzed according to gene frequency of different nationalities. Results None of HPA-b was found in Jingpo population samples and all the HPA-4 genotypes of Jingpo were aa. aa homozygosity was predominant except HPA-3. HPA-1a frequency of Achang was significantly different from that other populations in same study. HPA-2 frequency of Jingpo was significantly different from that other populations in our study.The MP of HPA-5 system in Han group was significantly lower than that in other ethnic groups. Platelet antibody-screening results showed that antibodies against-HPA3 system were the most in positive cases. Conclusion The distribution of HPA1-5 gene frequency in different ethnic groups in Yunnan has its own characteristics, and the influence of ethnic factors should be considered in the prevention, diagnosis and treatment of obstetric diseases.

Key words: Human platelet antigens, Gene polymorphism, Ethnic minorities