Abstract: Objective To detect HPA genotypes of blood samples from apheresis platelet donors in Hefei, Anhui Province,and calculate their distribution frequency in this region. Methods Multiplex PCR was used to characterize HPA1-6,10,15 and 21 genotypes of blood samples from 1181 apheresis platelet donors aged 18-55. Blood samples from 22 apheresis platelet donors aged 18-55 were tested for HPA genotyping using SBT direct sequencing. Results HPA-2, HPA-3 and HPA-15 were the most polymorphic in the Anhui population, and the frequencies of HPA alleles were as follows∶1a∶0.994 1,1b∶0.005 9,2a∶0.946 2,2b∶ 0.053 8,3a∶ 0.574 1,3b∶ 0.425 9,4a∶0.998 7,4b∶0.001 3,5a∶0.981 8,5b∶0.018 2,6a∶0.985 2,6b∶ 0.014 8,10a∶1.0,10b∶0.0,15a∶0.539 4、15b∶0.460 6,21a∶0.992 8、21b∶0.007 2. Conclusion The results of HPA genotyping are similar to those of SBT direct sequencing. The polymorphisms of HPA genotypes in Hefei,Anhui Province basically consist with those in other regions reported in the literature,but the distribution frequency of genotypes is specific to this region.

Key words: HPA genotyping, Multiple fluorescence PCR, SBT gene sequencing, Gene distribution frequency, Platelet donor database