Abstract: Objective To analyze the reference intervals of liver function index before blood transfusion in hospitalized newborns with different gestational ages and days-old,and complete the "reference value of liver function indexes" of hospitalized newborns. Methods This study adopted a multi-center retrospective survey method,taking 5 669 newborns admitted to 46 hospitals in China from January 1,2017 to June 30, 2018 as the research objects. According to a unified data collection table,the diagnosis and treatment data of hospitalized newborns were collected. The data were divided into three groups according to gestational age：<32 w group,32~36 w group,and ≥37 w group,and the liver function indicators of different days-old and gestational ages were analyzed. Results （1）The results of ALT showed that the median ALT of 0~28 days in the two groups of preterm infants was 5~8 and 8~11 U/L,respectively. The higher ALT of term infants was 11~22 U/L, which was statistically significant among different gestational age groups. ALT increased gradually with gestational age among the same days-old groups,and the ALT value of full-term infants was the highest. There were significant differences among all groups except 13~14 days of age. （2）TBIL and DBIL of newborns with different gestational age increased at first and then decreased with the increase of days-old. TBIL and DBIL were higher with the increase of gestational age in the same days-old group. （3）TP value of the three groups decreased 1~2 days after birth and then showed a gradual upward trend, and there were statistical significance in comparison with different days-old. TP increased with the increase of gestational age in the days-old. （4）ALB increased with the increase of gestational age in the same days-old group. Conclusion The reference range of liver function index is related to neonatal gestational age and days-old. Studying the range of liver function index of neonates with different gestational age and days-old is helpful to improve the accurate treatment of neonatal diseases.

Key words: Liver function, Neonatology, Multicenter study