Establishment and Analysis of the Database of CD36 Antigen-deficient Platelet Donors in Dongguan

doi:10.3969/j.issn.1671-2587.2025.04.009

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (4): 492-498.DOI: 10.3969/j.issn.1671-2587.2025.04.009

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Establishment and Analysis of the Database of CD36 Antigen-deficient Platelet Donors in Dongguan

HE Ziyi¹, HU Yingming¹, BEI Menghui¹, LIU Jing², JIE Xiaomei¹, CHEN Shaobin¹, ZHONG Chihui¹, LIANG Huaqin²

¹Dongguan Blood Center, Dongguan 523011, Guangdong;
²Guangzhou Blood Center, Guangzhou 5140410, Guangdong

Received:2025-02-18 Published:2025-08-22

Abstract

Abstract: Objective To establish a database of CD36 antigen-deficient platelet donors in Dongguan and analyze the characteristics. Methods Samples from 565 blood donors were tested for platelet CD36 antigen expression using flow cytometry. The gene sequences of 13 CD36-deficient samples and 187 samples from blood donors included in the database were analyzed using the third-generation gene sequencing (TGS). Results Flow cytometry detected CD36 deficiency in 13 cases, yielding a deficiency rate of 2.30% (13/565). Of these, 2 cases were type Ⅰ deficient (15.38%) and 11 cases were type Ⅱ deficient (84.62%). There was no significant difference in the rate of CD36 antigen deletion based on gender (P>0.05), ethnicity (Han vs. minority groups, P>0.05), or ABO blood group distribution (P>0.05). TGS revealed mutations in 7 of the 13 CD36 deficient samples, including 5 exons and 2 intron regions. Both type Ⅰ cases showed no detectable mutations, while all type Ⅱ cases exhibited heterozygous mutations. Additionally, 27 CD36 gene mutations were identified in 187 CD36 normal donors. The mutation rate in CD36 deficient donors (53.85%, 7/13) was significantly higher than that of CD36 normal donors (14.44%, 27/187) (P<0.05). Conclusion The frequency of platelet CD36 deficiency in Dongguan is higher than that reported in Chongqing but consists with findings from other Chinese regions. Establishing a serologically validated CD36-deficient platelet donor database in the area is recommended to enhance clinical transfusion safety.

Key words: Platelets, CD36, Gene, Blood donor, Platelet donor database

CLC Number:

R457.1

HE Ziyi, HU Yingming, BEI Menghui, LIU Jing, JIE Xiaomei, CHEN Shaobin, ZHONG Chihui, LIANG Huaqin. Establishment and Analysis of the Database of CD36 Antigen-deficient Platelet Donors in Dongguan[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(4): 492-498.

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Establishment and Analysis of the Database of CD36 Antigen-deficient Platelet Donors in Dongguan

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