Abstract: Objective We investigated the relevant indexes of routine umbilical cord blood and the mass percentage of various types of hemoglobin（HbA%,HbF%,and HbA₂%）of full~term newborns in Zhejiang Province to determine their reference value ranges,and therefore provide a reference for the screening of neonatal hemoglobinopathy and other diseases. Methods Blood routine and hemoglobin capillary electrophoresis were used to detect 1 788 cord blood donated by full~term healthy newborns. The percentile method was used to define the normal reference value ranges of bilateral 95% confidence intervals of WBC,RBC,Hb concentration,MCV,MCH,MCHC,HbA%,HbF and HbA₂%,and T~test or analysis of variance was used to judge the effects of gestational weeks and Hb concentration on HbA%,HbF% and HbA₂%. Results The reference values of WBC,RBC,Hb,MCV,MCH and MCHC in cord blood of full~term healthy newborns in Zhejiang Province were as follows：（9.7~23.4）×10⁹pieces/L,（3.5~5.0）×10¹²PCS/L,124~178 g/L,111~131 fl,33~39 pg/cell,and 280~315 g/L. The reference value range was 11.1%~32.1% in hemoglobin HbA%,67.5%~88.9% in HbF%,and 0~0.4% in HbA₂%. Gestational age and Hb concentration had effects on HbA%,HbF% and HbA₂%,but neonatal MCV,MCH,maternal age,neonatal gender and weight not. Conclusion The reference value ranges of related indexes of routine umbilical cord blood and hemoglobin capillary electrophoresis in newborns in Zhejiang Province can provide a basis for the diagnosis of diseases. Gestational weeks and Hb concentration may affect the mass percentage of HbA%,HbF% and HbA₂% of newborns.

Key words: Umbilical cord blood, Blood routine test, Capillary electrophoresis, Hemoglobinopathy