Abstract: ObjectiveTo compare the effect of blood group typing for ABO and RhD by using Metis-200 (Shenzhen Icahn) automatic blood analyzer and Xantus + Poseidon. MethodsTwo kinds of automatic blood analyzers were used to test 24 866 blood samplesblood interference, and comparative analysis. ResultsAccurate interpretation of the ABO blood group by the two analyzers was 100%. Metis-200 and Xantus dual robotics pipette joint Poseidon digital instrument presented seven and five cases of ABO blood group subtypes, respectively (P > 0.05 ); irregular antibodies were detected in 16 and 9 cases (P>0.05 ). 76 samples with different degrees of blood fat were examined by the two instruments for the anti-interference test, and the correct interpretation rate was 100%. Haemolysis interference test, when Hb concentration was less than or equal to 27 g/L, showed no negative impact on the interpretation of "UV" micro plate method. Conclusion