CD4+CD25+FOXP3+ Regulatory T cells in Asthmatic Mice

doi:10.3969/j.issn.1671-2587.2017.04.002

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2017, Vol. 19 ›› Issue (4): 317-320.DOI: 10.3969/j.issn.1671-2587.2017.04.002

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CD4+CD25+FOXP3+ Regulatory T cells in Asthmatic Mice

YANG Hui-hui, ZHANG Hen, SU Hong

Department of Epidemiology and Health Statistics,School of Public Health,Anhui Medical University,Hefei 230032

Received:2017-03-19 Online:2017-08-20 Published:2017-08-09

Abstract

Abstract: Objective To observe the change of CD4+CD25+FOXP3+ regulatory T cells （Tregs） in mouse model of asthma.Methods A total of 20 Balb/c female SPF-class mice were randomly divided into normal control group （n=10） and asthma model group （n=10）.The asthma model group was sensitized with 2% ovalbumin （OVA） by a combination of intraperitoneal injection to induce asthma.Mice of normal control group received normal salineinstead of OVAin equal volume.The counts of eosinophils in peripheral blood （PB）and bronchoalveolar lavage fluid （BALF） weredetected.The number of Tregs in mouse spleens was analyzed.Results The numbers of CD4+CD25+/CD4+（%）,CD4+FOXP3+/CD4+（%）,and CD4+CD25+FOXP3+/CD4+（%） in mouse spleens in asthma and normal control groups were 2.50±0.12 vs.16.64±2.11,3.92±1.23 vs.27.72±0.15,and 1.34±0.25 vs.15.60±0.62,respectively,showing significant differences （P<0.05）.Conclusion The proportion of CD4+CD25+FOXP3+ cells in asthma mice was decreased compared to controls,suggesting that tregsmay be involved in asthma pathogenesis.

Key words: Asthma, Regulatory T, Cell FOXP3

CLC Number:

R562.2⁺5R392.11

YANG Hui-hui, ZHANG Hen, SU Hong. CD4+CD25+FOXP3+ Regulatory T cells in Asthmatic Mice[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2017, 19(4): 317-320.

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CD4+CD25+FOXP3+ Regulatory T cells in Asthmatic Mice

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