• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2016, Vol. 18 ›› Issue (5): 491-494.DOI: 10.3969/j.issn.1671-2587.2016.05.029

• 技术与方法 • 上一篇    下一篇

不同冷冻温度、时间对冻干血小板回收率和残留水的影响*

吴学忠, 吕蓉, 吴君胜, 金惠新, 王震, 胡晓玉, 郭晓婕   

  1. 230032 合肥,安徽医科大学公共卫生学院（吴学忠）,安徽省血液中心（吴学忠,吕蓉,吴君胜,金惠新,王震,胡晓玉,郭晓婕）
  • 收稿日期:2016-02-09 出版日期:2016-10-20 发布日期:2017-05-19
  • 作者简介:吴学忠（1968-）,安徽霍邱人,副主任检验技师,硕士,主要从事血液检验及输血相关研究,（Tel）0551-65355093（E-mail）wuxuezhong2002@126.com。
  • 基金资助:
    *本课题受合肥市科技计划（自筹）项目（No.2014513181049）资助

The Effects of the Freeze-drying Temperature and Time on the Recovery and Residual Water in Lyophilized Platelets

WU Xue-zhong, LV Rong, WU Jun-sheng, et al   

  1. School of public health,Anhui Medical University, Hefei, 230032
  • Received:2016-02-09 Online:2016-10-20 Published:2017-05-19

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摘要： 目的 探讨不同冷冻温度、时间对冻干血小板回收率及残留水的影响。方法 依据温度、时间不同的冻干流程分3组（A组、B组和C组）,分别进行血小板冻干、复水试验。用干燥称重的方法检测冻干血小板残留水含量,比较不同冷冻温度、时间对冻干血小板回收率和残留水的差异,同时比较不同冷冻温度、时间冻干血小板复水后MPV、PDW的差异。结果 不同冷冻温度、时间条件下,冻干前与冻干复水后血小板MPV结果的差异均无统计学意义（P>0.05）;冻干前与复水后血小板PDW结果比较,A组和C组的差异均无统计学意义（P>0.05）,而B组冻干复水后血小板PDW高于冻干前血小板PDW,且差异有统计学意义（P<0.05）;A组、B组和C组冻干血小板复水回收率分别为（81.72±12.43）%、（81.40±8.79）%和（94.37±7.02）%,3组冻干血小板复水回收率的差异均无统计学意义（P>0.05）;A组、B组和C组冻干血小板残留水分别为（6.55±0.96）%、（7.10±1.20）%和（5.18±0.89）%,3组冻干血小板残留水含量的差异均无统计学意义（P>0.05）。结论 3组不同冻干温度、时间冻干血小板残留水、复水回收率相比,选择C组血小板冻干流程为后续研究血小板冻干程序,为血小板冻干流程的建立奠定基础。

关键词: 温度, 时间, 冻干血小板, 残留水, 回收率

Abstract: Objective To investigate the effect of varied freezing temperature and time on the recovery and residual water in the freeze-dried platelets. Method The freeze-drying processes of temperature and time were divided into three types (A, B and C). The platelets were freeze-dried and rehydrated using the three types of freeze-drying processes,respectively. The residual water content of the lyophilized platelets was detected with dry-weighing method.The differences of the recovery rate and residual water,and the MPV and PDW values in the freeze-dried platelets were compared. Result

The difference between freezing temperature/time for the freeze-drying platelets，the difference of the MPV values after the rehydration of freeze-dried platelets were not statistically significant （P>0.05）. The PDW value of freeze-dried platelets after rehydration between type A and type C showed no significant difference （P>0.05）, while that of type B was high （P<0.05）. The recovery rate of types A, B and C rehydration platelets was （81.72±12.43）%，（81.40±8.79）% and （94.37±7.02）%, respectively, with no significant difference among the three types （P>0.05）. The lyophilized platelets residual water contents in types A, B and C were （6.55±0.96）%，（7.10±1.20）%，and （5.18±0.89）%， respectively，with no significant difference among the three types （P>0.05）. Conclusion Based on the residual water and recovery of the freeze-dried platelets by using various processes，freeze-drying temperature and time should be optimized.

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