叶酸代谢相关酶基因多态性与不良孕产的关系研究

doi:10.3969/j.issn.1671-2587.2019.05.020

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (5): 514-516.DOI: 10.3969/j.issn.1671-2587.2019.05.020

叶酸代谢相关酶基因多态性与不良孕产的关系研究

叶芳, 黄婷婷, 徐徽, 蒋浏馨, 伊兴旭, 王宏志, 凡任芝

2300611 合肥,中国科学院合肥肿瘤医院（叶芳,黄婷婷,徐徽,蒋浏馨,伊兴旭,王宏志）;
中国科学技术大学第一附属医院（凡任芝）

收稿日期:2018-10-09 出版日期:2019-10-20 发布日期:2019-10-29
通讯作者: 凡任芝,男,副主任技师,硕士,主要从事临床检验诊断学质量管理控制,（E-mail）13705605248@163.com。
作者简介:叶芳（1980-）,女,安徽东至人,副主任技师,硕士,主要从事分子诊断学研究,（E-mail）764002645@qq.com。
基金资助:
*本课题受国家自然基金项目（No.31501171）; 安徽省自然基金项目（No.81872066）资助

Relationship between Folate Metabolism Related Enzyme Gene Polymorphism and Adverse Pregnancy Outcomes

YE Fang, HUANG Ting-ting, XU Hui, et al

Cancer Hospital,Chinese Academy of Sciences,Anhui,230001

Received:2018-10-09 Online:2019-10-20 Published:2019-10-29

摘要/Abstract

摘要： 目的分析探讨叶酸代谢相关酶基因多态性与不良孕产间的关系。方法选择2018年1月~2018年8月本院接收的不良孕产妇56例为研究组,另选同一时期正常生育产妇56例为对照组,两组均采用PCR法进行基因多态性检测,对比两组的基因型分布特点,并分析与不良孕产之间的关联性。结果研究组的MTHFR基因A1298C位点CC突变纯合型、C677T位点TT突变纯合型与MTRR基因A66G位点GG突变纯合型的基因频率均较对照组更高（P<0.05）;而研究组的MTHFR基因A1298C位点AA野生纯合型、C677T位点CC野生纯合型及MTRR基因A66G位点AA野生纯合型基因频率则较对照组更低（P<0.05）。结论不良孕产与叶酸代谢相关酶基因多态性之间关系密切。

关键词: 叶酸代谢相关酶, 基因多态性, 不良孕产关系研究

Abstract: Objective This study is to explore the relationship between folate metabolism related enzyme gene polymorphism and adverse pregnancy outcomes.Methods 56 unhealthy pregnant and lying-in women received in our hospital from January 2018 to August 2018 were selected as study group and 56 normal pregnant and lying-in women in the same period were selected as control group. PCR was used to detect gene polymorphism in both groups. The genotype distribution characteristics of the two groups were compared and the correlation between the two groups was analyzed.Results The frequencies of CC homozygous mutation,TT homozygous mutation at C677T locus and GG homozygous mutation at A66G locus of MTRR gene in the study group were higher than those in the control group （P<0.05）,while the frequencies of AA wild homozygous at A1298C locus of MTHFR gene,CC wild homozygous at C677T locus and AA at A66G locus of MTRR gene in the study group were higher than those in the control group （ The frequency of wild homozygous genes was lower than that of the control group （P<0.05）.Conclusion There is a close correlation between adverse pregnancy outcomes and folate metabolism related enzyme gene polymorphism.

Key words: Folate metabolism related enzyme gene polymorphism, Adverse pregnancy outcomes, Relationship study

中图分类号:

R392.11 R714.2

叶芳, 黄婷婷, 徐徽, 蒋浏馨, 伊兴旭, 王宏志, 凡任芝. 叶酸代谢相关酶基因多态性与不良孕产的关系研究[J]. 临床输血与检验, 2019, 21(5): 514-516.

YE Fang, HUANG Ting-ting, XU Hui, et al. Relationship between Folate Metabolism Related Enzyme Gene Polymorphism and Adverse Pregnancy Outcomes[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2019, 21(5): 514-516.

参考文献

1 潘伟,冯晓萍,俞磊. 叶酸代谢相关酶系基因多态性与不良孕史的相关性研究[J]. 中国妇产科临床杂志,2017,18(4):332-336.
2 Merviel P,Cabry R,Lourdel E,et al. Comparison of twopreventive-treatments for patients with recurrent miscarriages carrying a C677T-methylenetetrahydrofolate reductase mutation: 5-year experience[J]. J Int Med Res,2017,45(6):1720-1730.
3 Chang H,Zhang T,Zhang Z,et al. Tissue-specific distributionof aberrant DNA methylation associated with maternal low-folate status in human neural tube defects[J].J Nutr Biochem,2011,22(12):1172-1177.
4 谢晓媛,张颖,辛力,等. 叶酸代谢酶MTHFR、MTRR基因多态性与原因不明复发性流产的关系[J]. 天津医药,2016,44(10):1243-1246.
5 肖鸽飞,孟小军,胡玲玲,等. 孕妇叶酸代谢相关基因与胎儿先天缺陷的相关性研究[J]. 中国医师杂志,2016,18(7):1021-1024.
6 Hui F,Su R,Song Y,et al. Positive correlation between enhancedexpression of TLR4/MYD88/NF-KB with insulin resistence in plancetae of gestational diabetes mellitus[J]. PLOS One,2016,11(6): e0157185.
7 张仪,有风芝,秦奇,等. MTHFR基因多态性及叶酸摄入量与不良孕产史关系的研究[J].医药论坛杂志,2017,38(1):66-69.
8 Guo Q N,Wang H D,Tie L Z,et al. Parental Genetic Variants, MTHFR 677>T and MTRR 66A>G,Associated Differently with Fetal Congenital Heart Defect[J].Biomed Res Int,2017(4):3043476-3043477.
9 姚涛,李三华,宋庆高. 叶酸代谢关键酶基因多态性与非综合征性唇腭裂相关性研究进展[J]. 医学研究生学报,2017,30(3):310-314.
10 南欣荣,任一雄,李瑞芳,等. 叶酸代谢相关基因多态性与山西地区非综合征性唇腭裂的相关性研究[J].实用口腔医学杂志,2015,31(1):96-100.
11 Xie X Y,Zhang Y,Xin L,et al.Relationship of folate metabolism-related enzymes MTHFR and MTRR gene polymorphismswith-unexplained recurrent spontaneous abortion[J]. International-Journalof Clinical and Experimental Pathology,2017,10(3):1936-2625.
12 Zidan H E,Rezk N A,Mohammed D. MTHFR C677T and A1298C gene polymorphisand their relation to homocysteine level in Egyptianchildren with congenital heart disease[J].Gene,2013,529(1):119-124.
13 Stangler Herode S,Zagradinik B,Erjaveckerget A,et al. MTHFRC677T and A1298C genotypes and haplotypes in Slovenian coupleswith unespained infertility problems and in embyyonic tissues fromspontaneos abortions[J].Balkan J Med Genet,2013,16(1):31-40.
14 刘晓玲. 叶酸代谢相关酶基因多态性与不良孕产的临床关系分析[J]. 深圳中西医结合杂志,2016,26(12):26-27.
15 陆继红,施晓艳,万金华.分析叶酸代谢相关酶基因多态性与不良孕产的临床关系[J].中国实用医药,2017,12(16):88-89.
16 高洁,王涛,肖海,等. 叶酸代谢基因MTHFR及MTRR多态性与河南地区复发性流产的相关性分析[J].临床医学,2015,42(11):1-4.
17 Yenicesu G I,Cetin M,Ozdemir O,et al.A Prospective Case-Control Study Analyzes 12 Thrombophilic Gene Mutations in Turkish Couples with Recurrent Pregnancy Loss[J].Am J Reprod Immunol,2010,63(2):126-136.
18 Wujcicka W,Paradowska E,Studzifska M,et al. Toll-like receptorsgenes polymorphisms and the occurrence of HCMV infection amongpregnant women[J]. Virology J,2017,14(1):64.
19 Hocker A D,Stokes J A,Powell F L,et al. The impact of inflammationon respiratory plasticity[J].Exp Neurol,2017,287(Pt2):243-253.
20 李翠苑,甘伟红,古丽巴哈,等. 个体化营养指导对双胎妊娠的结局影响分析[J].2016,47(3):81-83.
21 杨玉清,张燕,鲁衍强,等.银川市回族女性叶酸相关基因多态性与不良妊娠的相关性研究[J].中国妇幼保健,2017,32(19):4758-4760.

[1]	成枫, 许宏, 肖淑欣, 赵洪国. HPA-1-6/17基因多态性与青岛市血小板减少症遗传易感性的关系分析[J]. 临床输血与检验, 2020, 22(5): 521-526.
[2]	何智. 乌鲁木齐地区哈萨克族献血人群HPA-15基因多态性分析[J]. 临床输血与检验, 2019, 21(2): 152-154.
[3]	赵佳, 苏蔓, 何路军, 戚海, 李茵, 胡光磊, 王远花, 钱明明, 李醒, 张蓓, 王振雷. 河北地区汉族人群KIR基因多态性研究[J]. 临床输血与检验, 2017, 19(6): 566-569.
[4]	汪波, 范利平, 史德宝, 程筱雯, 李洁, 徐元宏. 安徽地区汉族孕妇人群MTHFR基因多态性研究^*[J]. 临床输血与检验, 2017, 19(6): 581-584.
[5]	刘爱胜, 郭龙华, 文艳, 刘小君, 林丽云, 房笃智. GSTM1和CYP2E1基因多态性与非小细胞肺癌遗传易感性的相关性研究^*[J]. 临床输血与检验, 2017, 19(3): 260-265.
[6]	林红, 邵雷. 江苏地区汉族人群HLA-E基因多态性分析^*[J]. 临床输血与检验, 2017, 19(2): 173-175.

叶酸代谢相关酶基因多态性与不良孕产的关系研究

Relationship between Folate Metabolism Related Enzyme Gene Polymorphism and Adverse Pregnancy Outcomes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

关于本刊

作者中心

在线期刊

访问统计

联系我们