细胞色素P450基因单核苷酸多态性与乙肝肝硬化易感性研究*

doi:10.3969/j.issn.1671-2587.2023.06.016

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (6): 806-812.DOI: 10.3969/j.issn.1671-2587.2023.06.016

细胞色素P450基因单核苷酸多态性与乙肝肝硬化易感性研究^*

魏华, 杨龙飞, 李娜, 陈洁, 富文达, 穆士杰

空军军医大学唐都医院输血科,西安 710038

收稿日期:2023-08-15 出版日期:2023-12-20 发布日期:2024-01-15
通讯作者: 穆士杰,主要从事临床输血医学及血液治疗研究,（E-mail）musj1963@fmmu.edu.cn。
作者简介:魏华,主要从事血型基因组学研究,（E-mail）tangduweihua@163.com。
基金资助:
*本课题受空军军医大学第二附属医院青年自主创新科学基金项目（No.2023BTDQN023）资助

Association of SNP of Cytochrome P450 with Susceptibility to Hepatitis B Virus Cirrhosis

WEI Hua, YANG Longfei, LI Na, et al

Department of Transfusion Medicine, Tangdu Hospital, Air Force Medical University, Xi'an 710069

Received:2023-08-15 Online:2023-12-20 Published:2024-01-15

摘要/Abstract

摘要： 目的初步探索细胞色素P450基因SNP位点多态性是否与乙肝肝硬化的发生风险相关。方法本研究通过文献调研后从生物信息学数据库筛选细胞色素P450基因上的10个位点,收集乙肝肝硬化组255例及健康组160例样本通过测序进行基因分型。应用Logistic回归等统计学分析验证基因SNP位点与乙肝肝硬化易感性。结果所有的SNP位点基因型分布均符合Hardy-Weinberg平衡。χ²检验显示10个位点中仅rs3758581位点基因型及等位基因分布在乙肝肝硬化组与健康组间,差异有统计学意义。调整协变量的Logistic回归分析可见,rs3758581中共显性、加性、显性及超显性模型提示GA基因型患乙肝肝硬化风险升高。结论细胞色素P450基因中rs3758581位点杂合突变GA基因型可能会增加乙肝肝硬化风险。

关键词: CYP450基因, 单核苷酸多态性（SNP）, 乙肝肝硬化, rs3758581位点

Abstract: Objective To investigate whether the SNP of cytochrome P450 gene is associated with a risk of liver cirrhosis induced by hepatitis B virus (HBV). Methods We screened 10 loci on cytochrome P450 genes from bioinformatics database after literature research, and then collected 255 samples in HBV-related cirrhosis group and 160 samples in healthy group for genotyping by sequencing. Logistic regression and other statistical analyses were used to analyze the relationship between SNP and susceptibility to HBV-related cirrhosis. Results The genotype distribution of all SNP loci was in Hardy-Weinberg equilibrium. The chi-square test showed that only rs3758581 genotype and allele distribution at 10 loci were significantly different between HBV-related cirrhosis group and healthy group. Logistic regression analysis showed that the codominant, additive, dominant and overdominance models at rs3758581 suggested patients with GA genotype had an increased risk of HBV-related cirrhosis. Conclusion The GA genotype heterozygous mutation at rs3758581 on cytochrome P450 gene may increase the risk of HBV-related cirrhosis.

Key words: CYP450 gene, Single-nucleotide polymorphism, HBV-related cirrhosis, rs3758581

中图分类号:

R657.3⁺1

魏华, 杨龙飞, 李娜, 陈洁, 富文达, 穆士杰. 细胞色素P450基因单核苷酸多态性与乙肝肝硬化易感性研究^*[J]. 临床输血与检验, 2023, 25(6): 806-812.

WEI Hua, YANG Longfei, LI Na, et al. Association of SNP of Cytochrome P450 with Susceptibility to Hepatitis B Virus Cirrhosis[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(6): 806-812.

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细胞色素P450基因单核苷酸多态性与乙肝肝硬化易感性研究^*

Association of SNP of Cytochrome P450 with Susceptibility to Hepatitis B Virus Cirrhosis

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