1例由抗McCb抗体引起的新生儿溶血病

doi:10.3969/j.issn.1671-2587.2025.06.017

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (6): 849-853.DOI: 10.3969/j.issn.1671-2587.2025.06.017

1例由抗McCb抗体引起的新生儿溶血病

张钰¹, 丁蔚², 何鑫³, 何红红⁴, 何成涛¹, 沈伟²

¹南京红十字血液中心研究室,江苏南京 210003;
²上海市血液中心血型参比实验室,上海 200051;
³上海交通大学医学院附属新华医院输血科,上海 200092;
⁴苏州市中心血站检验科,江苏苏州 215002

收稿日期:2025-02-18 出版日期:2025-12-20 发布日期:2025-12-24
通讯作者: 沈伟,主要从事输血技术及血液免疫学方面研究,E-mail：eqas@sbc.org.cn。
作者简介:张钰,主要从事输血免疫学研究,E-mail：624702724@qq.com。并列第一作者：丁蔚,主要从事质量控制及培训工作,E-mail：dingwei7911@163.com。

A Case of Hemolytic Disease of the Fetus and Newborn due to Anti-McCb

ZHANG Yu¹, DING Wei², HE Xin³, HE Honghong⁴, HE Chengtao¹, SHEN Wei²

¹Nanjing Red Cross Blood Center,Nanjing 210003;
²Blood Group Reference Laboratory, Shanghai Blood Center, Shanghai 200051;
³Department of Blood Transfusion, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092;
⁴Department of Clinical Laboratory, Suzhou Central Blood Station, Suzhou 215002

Received:2025-02-18 Online:2025-12-20 Published:2025-12-24

摘要/Abstract

摘要： 目的探讨低频抗原抗体抗McCb检测方法在一例新生儿溶血病患儿中的应用。方法本案例因患儿出生24 h后出现黄疸,怀疑为ABO血型以外的意外抗体导致的新生儿溶血病,其母亲血清与谱细胞反应均为阴性,须确认父亲红细胞上是否表达某种低频抗原。因此将母亲血清分别与父亲及患儿红细胞进行反应,反应结果均为阳性,说明母亲血清中存在针对父亲及患儿红细胞上某种低频抗原的抗体。将不同的酶或化学试剂分别处理父亲的红细胞,造成高频抗原或低频抗原在不同酶的反应格局差异,进而进一步鉴定特异性的抗原和抗体。结果高度怀疑为抗McCb抗体引起的新生儿溶血病。结论 PEG增强试验和酶处理技术有助于低频抗原抗体的检出,后续还需分子生物学方法进行确认。

关键词: 低频抗原抗体, Knops血型系统, 酶处理细胞, 抗McCb

Abstract: Objective To explore the detection method of low-frequency antigen antibody anti-McCb in a case of hemolytic disease of the fetus and newborn. Methods In this case, the infant developed jaundice 24 hours after birth, which raised suspicion of neonatal hemolytic disease caused by unexpected antibodies other than those of the ABO blood group. The mother's serum showed no reaction with panel cells, so it was necessary to confirm whether the father's red blood cells expressed a certain low-frequency antigen. Therefore, the mother's serum was reacted separately with the father's and the infant's red blood cells. The positive results indicated the presence of antibodies in the mother's serum targeting a certain low-frequency antigen on the father's and the infant's red blood cells. Different enzymes or chemical reagents were used to treat the father's red blood cells, creating different reaction patterns for high-frequency and low-frequency antigens under various enzymes, thereby further identifying the specific antigens and antibodies. Results It is highly suspected that the neonatal hemolytic disease is caused by anti-McCb antibodies. Conclusion PEG enhancement test and enzyme treatment technology are helpful to the detection of anti-low frequency antigen antibodies, and molecular biological methods are still needed for confirmation.

Key words: Low frequency antigenic antibody, Knops blood group system, Enzyme treated cell, Anti-McCb

中图分类号:

R722.18

张钰, 丁蔚, 何鑫, 何红红, 何成涛, 沈伟. 1例由抗McCb抗体引起的新生儿溶血病[J]. 临床输血与检验, 2025, 27(6): 849-853.

ZHANG Yu, DING Wei, HE Xin, HE Honghong, HE Chengtao, SHEN Wei. A Case of Hemolytic Disease of the Fetus and Newborn due to Anti-McCb[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(6): 849-853.

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1例由抗McCb抗体引起的新生儿溶血病

A Case of Hemolytic Disease of the Fetus and Newborn due to Anti-McCb

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