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临床输血与检验

临床输血与检验 ›› 2022, Vol. 24 ›› Issue (4): 432-437.DOI: 10.3969/j.issn.1671-2587.2022.04.005

• 临床输血 • 上一篇    下一篇

探索不同Rh表型细胞与一种抗CD47单克隆抗体TJC4结合的血清学特征

权军辉, 张明刚, 吴姣姣, 赵景杰, 韩梅宁   

  1. 710004 西安交通大学附属第二医院输血科
  • 收稿日期:2021-09-20 出版日期:2022-08-20 发布日期:2022-08-19
  • 作者简介:权军辉（1987-）,男,主管技师,主要从事临床输血及输血相关实验研究,（E-mail）815746613@qq.com。

Exploring the Serological Characteristics of Cells with Different Rh Phenotypes Bounded an anti-CD47 McAb, TJC4

QUAN Jun-hui, ZHANG Ming-gang, WU Jiao-jiao, et al   

  1. Department of Blood Transfusion, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004
  • Received:2021-09-20 Online:2022-08-20 Published:2022-08-19

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摘要： 目的 1）探索不同Rh表型细胞与抗CD47的IgG4单克隆抗体TJC4结合的血清学特征;2）明确TJC4对输血相容性试验干扰消除的处理策略。方法 抽取EDTA-K2抗凝非肿瘤且无急慢性基础疾病A型Rh表型分别为CCDee、CcDEe、CcDee、ccDEE、ccDEe、ccdee、Ccdee、CCdee患者静脉血各5 mL,运用微柱凝胶法、盐水法、聚凝胺法分别做DAT（直接抗球蛋白试验）、IAT（间接抗球蛋白试验）、交叉配血等输血相容性试验,检测TJC4体外致敏、独特型抗体ID-ab中和、Immucor AHG干扰去除情况。结果 1）该研究纳入所有Rh表型细胞被TJC4致敏后盐水、聚凝胺、微柱凝胶三种方法DAT、IAT、交叉配血试验均呈不同凝集强度的阳性反应,且该干扰均能够通过Immucor AHG LISS增强剂法消除;2）独特型抗体ID-ab中和试验去除CD47单抗对ccDEe和ccdee表型血清学实验的干扰需加大剂量。结论 1）不同Rh表型细胞与TJC4结合后不同输血相容性试验方法呈现不同的血清学差异;2）独特型抗体ID-ab中和试验及缺乏IgG4亚型检测功能的Immucor AHG LISS增强剂法均可消除CD47单抗所致的血清学干扰,但不同Rh表型细胞前者存在剂量差异。

关键词: Rh表型, TJC4, 血清学特征

Abstract: Objective 1）To explore the serological characteristics of cells with different Rh phenotypes bound to the anti-CD47 IgG4 monoclonal antibody TJC4;2）To clarify the treatment strategy of TJC4 to eliminate the interference of blood transfusion compatibility test. Method 5 mL of venous blood from patients with EDTA-K2 anticoagulation, non-tumor and no acute or chronic underlying diseases with Rh phenotypes of CCDee, CcDEe, CcDee, ccDEE, ccDEe, ccdee, Ccdee, and CCdee were collected, using microcolumn gel method, saline Blood transfusion compatibility tests such as DAT (direct antiglobulin test), IAT (indirect antiglobulin test), and cross-matching blood were respectively performed by the method and polybrene method to detect TJC4 in vitro sensitization and idiotype antibody ID-ab neutralization. , Immucor AHG interference removal.Results 1)All Rh phenotype cells sensitized by TJC4 showed positive reaction of DAT, IAT and cross-matching with varying intensity of agglutination. The interference can be eliminated by using the Immucor AHG LISS method. 2)In order to remove the interference of CD47 McAb to ccDEe and ccdee phenotypes, higher doses should be used in idiotypic antibody ID-ab neutralization test.Conclusion 1)In this study, various blood transfusion compatibility test methods with different Rh phenotypes combined with TJC4 were used and showed notable serological differences. 2)The idiotypic antibody ID-ab neutralization test and the Immucor AHG LISS enhancer assay lacking IgG4 subtype detection can eliminate the serological interference caused by CD47 McAb. However, there were dose differences among the different Rh phenotypes.

Key words: Rh phenotype, TJC4, Serological characteristics

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