血小板储存：解码pri-miR-223表达和代谢变化*

doi:10.3969/j.issn.1671-2587.2025.01.003

临床输血与检验 ›› 2025, Vol. 27 ›› Issue (1): 21-28.DOI: 10.3969/j.issn.1671-2587.2025.01.003

血小板储存：解码pri-miR-223表达和代谢变化^*

苏晓敏, 徐华, 张国权, 王锦, 马超锋

陕西省血液中心,陕西西安 710061

收稿日期:2024-09-23 发布日期:2025-02-25
通讯作者: 马超锋,主要从事病原生物学和输血医学相关研究工作,（E-mail）mark7447@xiancdc.cn。
作者简介:苏晓敏,主要从事输血医学和血液成分研究工作,（E-mail）sufeichi112@163.com。
基金资助:
*本课题受西安市科技局医学研究一般研究项目（No.23YXYJ0076）、西安市卫生健康委员会科研项目一般研究项目（No.2022yb07）、中国输血协会威高科研基金面上资助项目（No.CSBT-WG-2022-02）、西安市卫生健康委员会一般研究项目（No.2023yb48）资助

Platelet Storage: Decoding Pri-miR-223 Expression and Metabolic Alterations

SU Xiaomin, XU Hua, ZHANG Guoquan, WANG Jin, MA Chaofeng

Shaanxi Blood Center, Xi'an 710061

Received:2024-09-23 Published:2025-02-25

摘要/Abstract

摘要： 目的探讨血小板储存过程中伴随储存时间延长pri-mioroRNA-223的表达变化及意义。方法使用血细胞分析仪检测不同保存时间（1、3、5 d）的血小板质量,并进行代谢组学检测。同时,建立液滴式数字PCR（droplet digital PCR, ddPCR）方法,对其敏感性、重复性进行评价。用ddPCR检测血小板样本不同储存时间的pri-miR-223表达量,分析其对血小板储存损伤（PSL）的影响及意义。结果随着保存时间的延长,血小板不同保存时间的血小板计数（PLT）、血小板比例（P-LCR）、平均血小板体积（MPV）和血小板分布宽度（PDW）差异均无统计学意义（P>0.05）。而代谢组学研究共筛选出955种代谢产物,其中54种代谢产物上调,6种代谢产物下调。所建立的ddPCR方法特异性好,敏感性高,标准曲线表现出良好的线性相关性（Y=0.973 6X+0.083 2）,R²值为0.998 4,线性动态范围灵敏度为3.3×10⁰~1.9×10⁵拷贝/μL。ddPCR结果显示第3、5天样本中pri-miR-223表达量比第1天明显降低。结论随着血小板体外贮存时间的延长,对PLT、P-LCR、MPV和PDW没有影响,储存期末部分代谢产物改变,且通过ddPCR检测发现pri-miR-223表达量降低,这种代谢变化可能与pri-miR-223的表达调控有关。

关键词: 血小板储存, 代谢组学, 数字PCR, pri-miR-223

Abstract: Objective To investigate the expression changes and significance of pri-miR-223 associated with prolonged storage time during platelet storage. Methods Blood cell analyzer was used to detect platelet mass at different preservation times (1, 3, 5 days), and metabolomic analysis was performed. The Droplet Digital PCR (ddPCR) method was developed to evaluate its sensitivity and reproducibility. The ddPCR was used to detect the expression level of pri-miR-223 in platelet at different storage times, and to analyze the effect and significance of PRI-Mir-223 on platelet storage lesion (PSL). Results There were no significant differences in platelet count (PLT), platelet-large cell ratio (P-LCR), mean platelet volume (MPV) and platelet distribution width (PDW) among different preservation times with extended times (P>0.05). A total of 955 metabolites were identified by metabolomics, of which 54 metabolites were up-regulated and 6 metabolites were down-regulated. The developed ddPCR method showed good specificity and high sensitivity. The standard curve showed good linear correlation (Y=0.973 6 X+0.083 2). The R² value was 0.9984, and linear dynamic range sensitivity was 3.3×10⁰~1.9×10⁵ copies /μL. The ddPCR results showed that the expression level of pri-miR-223 on day 3 and 5 was significantly lower than that on day 1. Conclusion With extended storage time, PLT, P-LCR, MPV, and PDW were not affected, and some metabolites were changed at the end of the storage period, and ddPCR analysis showed the expression level of pri-miR-223 decreased, which may be related with the regulation of pri-miR-223 expression.

Key words: Platelet storage, Metabolomics, Digital PCR, pri-miR-223

中图分类号:

R457

苏晓敏, 徐华, 张国权, 王锦, 马超锋. 血小板储存：解码pri-miR-223表达和代谢变化^*[J]. 临床输血与检验, 2025, 27(1): 21-28.

SU Xiaomin, XU Hua, ZHANG Guoquan, WANG Jin, MA Chaofeng. Platelet Storage: Decoding Pri-miR-223 Expression and Metabolic Alterations[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(1): 21-28.

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血小板储存：解码pri-miR-223表达和代谢变化^*

Platelet Storage: Decoding Pri-miR-223 Expression and Metabolic Alterations

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