LncRNA SNHG9在正常机采血小板储存中表达变化及意义

doi:10.3969/j.issn.1671-2587.2023.03.004

临床输血与检验 ›› 2023, Vol. 25 ›› Issue (3): 310-315.DOI: 10.3969/j.issn.1671-2587.2023.03.004

LncRNA SNHG9在正常机采血小板储存中表达变化及意义

张文娟, 段勇, 纪玉强, 冯娜, 兰静, 景媛媛

710061 西安,陕西省血液中心（张文娟,段勇,冯娜,兰静,景媛媛）; 西安市第一医院中心实验室（纪玉强）

收稿日期:2023-03-18 发布日期:2023-07-10
通讯作者: 景媛媛,副主任技师,博士,主要从事免疫血液学和经血传播疾病的发病机制方面的研究,（E-mail）yuanyuanjing1010@126.com。
作者简介:张文娟,主管技师,博士,主要从事长链非编码RNA与血小板储存损伤的相关性研究,（E-mail）juner0705@163.com。
基金资助:
^*本课题受陕西省重点研发计划项目（No.2021SF-151）（No.2022SF-548）、西安市科技计划项目（No.22YXYJ0050）（No.21YXYJ0028）资助

LncRNA SNHG9 in Apheresis Platelets Following Storage

ZHANG Wenjuan, DUAN Yong, JI Yuqiang, et al

Shaanxi blood center, Xi'an 710061

Received:2023-03-18 Published:2023-07-10

摘要/Abstract

摘要： 目的探讨正常机采血小板储存过程中伴随储存时间延长长链非编码RNA（lncRNA）小核仁RNA宿主基因9（SNHG9）的表达变化及意义。方法收集符合临床血小板输注治疗标准的正常机采血小板32份,保存于专用（22±2）℃恒温振荡保存箱内,在不同储存时间（1、3、5、7、9 d）通过外观、血小板计数、红细胞残留量计数、白细胞残余量计数、pH值及细菌培养检测血小板质量;用实时荧光定量PCR（qRT-PCR）检测SNHG9在同一机采血小板样本不同储存时间的表达量,分析其对血小板储存损伤（PSL）的影响及意义。结果在第1、3、5、7、9天SNHG9表达量伴随储存时间延长逐渐增高,数据总体存在统计学差异（H=93.073,P<0.001）,差异有统计学意义（P<0.05）。进一步对不同天数之间SNHG9表达量进行两两多重比较,第1天与第3天校正后的P值是0.144,差异无统计学意义（P>0.05）;第1天和第5天,第1天和第7天,第1天和第9天校正后的P值均小于0.001,差异有统计学意义（P<0.05）;第3天和第5天（P=0.031）,第3天和第7天,第3天和第9天（P<0.001）校正后差异有统计学意义。结论用于临床治疗的正常机采血小板伴随储存时间延长,第5、7、9天样本中SNHG9表达量比第1天明显增高,SNHG9对血小板储存条件敏感,有可能成为PSL潜在的生物学标记物。

关键词: 机采血小板, 长链非编码RNA, 小核仁RNA宿主基因9, 血小板储存损伤

Abstract: Objective To investigate the expression changes of long noncoding RNA (lncRNA) small nucleolar RNA host gene 9 (SNHG9) with extended storage during apheresis platelet storage. Methods A total of 32 samples of apheresis platelets that met the indications for platelet transfusion were collected and stored at （22±2）℃ with gentle agitation. Platelets were measured by appearance, platelet count, red blood cell residual count, white blood cell residual count, pH value and bacterial culture in different storage times (1, 3, 5, 7, and 9 days). The expression levels of lncRNA SNHG9 in different storage times were detected by quantitative real-time PCR (qRT-PCR) and the expressions of SNHG9 on platelet storage lesion (PSL) were analyzed. Results The expressions of SNHG9 with extended storage at the 1st, 3rd, 5th, 7th and 9th days were gradually increased. There was a statistical difference (H=93.073, P<0.001), with a statistical significance (P<0.05). SNHG9 expressions at different days were further compared by pairwise multiple comparisons. The corrected P value at the 1st and 3rd day was 0.144 with no statistical significance (P>0.05). The corrected P values at the 1st and 5th, 1st and 7th, 1st and 9th day were less than 0.001 with statistical significance (P<0.05). Statistically significant differences were corrected for on 3rd and 5th day (P=0.031), 3rd and 7th day (P<0.001), 3rd and 9th day (P<0.001). Conclusion With extended storage, SNHG9 expressions in apheresis platelets stored at the 5th, 7th, and 9th day were significantly higher than that at the 1st day. SNHG9 is sensitive to platelet storage condition, and may be a potential biomarker for PSL.

Key words: Apheresis platelets, LncRNA, SNHG9, Platelet storage lesion

中图分类号:

R331.1⁺43

张文娟, 段勇, 纪玉强, 冯娜, 兰静, 景媛媛. LncRNA SNHG9在正常机采血小板储存中表达变化及意义[J]. 临床输血与检验, 2023, 25(3): 310-315.

ZHANG Wenjuan, DUAN Yong, JI Yuqiang, et al. LncRNA SNHG9 in Apheresis Platelets Following Storage[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(3): 310-315.

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LncRNA SNHG9在正常机采血小板储存中表达变化及意义

LncRNA SNHG9 in Apheresis Platelets Following Storage

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