PRP对人子宫内膜基质细胞增殖和迁移的影响及关键基因CDCA8的筛选*

doi:10.3969/j.issn.1671-2587.2026.01.007

临床输血与检验 ›› 2026, Vol. 28 ›› Issue (1): 44-51.DOI: 10.3969/j.issn.1671-2587.2026.01.007

PRP对人子宫内膜基质细胞增殖和迁移的影响及关键基因CDCA8的筛选^*

李健, 赵燕, 许静静, 孙悦, 徐佟, 常洪劲

济宁医学院附属医院，山东济宁 272000

收稿日期:2025-07-10 发布日期:2026-02-13
通讯作者: 常洪劲，主要从事PRP专项治疗、临床精准化输血及质量安全体系建设等相关工作，（E-mail）changhongjin@126.com。
作者简介:李健，主要从事临床输血治疗及疑难血型、疑难交叉配血等研究，（E-mail）lijian_edu@163.com。
基金资助:
*本课题受济宁医学院附属医院“苗圃”科研计划（No.MP-MS-2023-09）、山东省妇幼保健协会科研项目（No.SFYXH-2023Y032）、济宁市重点研发计划项目（No.2023YXNS094）资助

Effects of Platelet-rich Plasma on the Proliferation and Migration of Human Endometrial Stromal Cells and Screening of the Key Gene of CDCA8

LI Jian, ZHAO Yan, XU Jingjing, SUN Yue, XU TONG, CHANG Hongjin

Affiliated Hospital of Jining Medical University, Jining, Shandong 272000

Received:2025-07-10 Published:2026-02-13

摘要/Abstract

摘要： 目的本研究旨在探讨富血小板血浆（platelet-rich plasma, PRP）对永生化人子宫内膜基质细胞（immortalized human endometrial stromal cells, iehESCs）增殖和迁移的影响，确定PRP最佳浓度并筛选PRP促进细胞增殖和迁移功能的关键基因。方法本研究采用不同浓度PRP处理iehESCs细胞，通过细胞计数试剂盒-8（cell counting kit-8, CCK-8）和Transwell迁移实验评估细胞增殖和迁移能力，转录组测序分析差异表达基因，然后通过逆转录实时荧光定量聚合酶链式反应（reverse transcription quantitative real-time polymerase chain reaction, RT-qPCR）和蛋白质印迹法（western blotting）验证关键基因的表达。结果 5%和10% PRP组显著促进了iehESCs的增殖和迁移，而15% PRP组无显著促进作用。转录组测序结果显示，5% PRP组有1 032个差异表达基因。通过Gene Ontology（GO）和Kyoto Encyclopedia of Genes and Genomes（KEGG）通路富集分析，筛选出12个上调基因和3个下调基因，经验证CDCA8（cell division cycle associated 8, CDCA8）在mRNA和蛋白水平表达均上调。结论低浓度的PRP（5%和10%）能够显著促进iehESCs的增殖和迁移，而高浓度的PRP（15%）则抑制了细胞功能。PRP可通过上调CDCA8参与细胞周期进而促进iehESCs的增殖和迁移。

关键词: 富血小板血浆（PRP）, 人子宫内膜基质细胞, 细胞增殖, 细胞迁移, CDCA8

Abstract: Objective This study aimed to investigate the effects of platelet-rich plasma (PRP) on the proliferation and migration of immortalized human endometrial stromal cells (iehESCs), to determine the optimal concentration of PRP, and to identify the key gene mediating PRP-induced enhancement of cell proliferation and migration. Methods iehESCs were exposed to graded PRP concentrations. Cell proliferation and migratory capacity were assessed by CCK-8 assay and Transwell migration assay, respectively. Transcriptome profiling was performed to screen differentially expressed genes (DEGs), and the findings were validated by quantitative RT-qPCR and Western blot. Results PRP at 5% and 10% significantly enhanced both proliferation and migration of iehESCs, whereas 15% PRP showed no stimulatory effect. Transcriptomic analysis revealed 1 032 DEGs between the 5% PRP group and control group. GO and KEGG enrichment analyses highlighted 12 up-regulated and 3 down-regulated genes. Among them, CDCA8 (cell division cycle associated 8) was markedly up-regulated at both the mRNA and protein levels. Conclusion Low concentrations of PRP (5%~10%) promote iehESC proliferation and migration, whereas high concentrations (15%) are ineffective. The beneficial effects of PRP are, at least partially, mediated by up-regulation of CDCA8, which facilitates cell-cycle progression.

Key words: Platelet-rich plasma (PRP), Human endometrial stromal cells, Cell proliferation, Cell migration CDCA8

中图分类号:

R711.74R329.2

李健, 赵燕, 许静静, 孙悦, 徐佟, 常洪劲. PRP对人子宫内膜基质细胞增殖和迁移的影响及关键基因CDCA8的筛选^*[J]. 临床输血与检验, 2026, 28(1): 44-51.

LI Jian, ZHAO Yan, XU Jingjing, SUN Yue, XU TONG, CHANG Hongjin. Effects of Platelet-rich Plasma on the Proliferation and Migration of Human Endometrial Stromal Cells and Screening of the Key Gene of CDCA8[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(1): 44-51.

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PRP对人子宫内膜基质细胞增殖和迁移的影响及关键基因CDCA8的筛选^*

Effects of Platelet-rich Plasma on the Proliferation and Migration of Human Endometrial Stromal Cells and Screening of the Key Gene of CDCA8

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