• 中国科学论文统计源期刊
  • 中国科技核心期刊
  • 美国化学文摘(CA)来源期刊
  • 日本科学技术振兴机构数据库(JST)
临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (1): 89-91.DOI: 10.3969/j.issn.1671-2587.2018.01.030

• 临床检验 • 上一篇    下一篇

B群链球菌两种检测方法的比较

李金丽, 宋晓光   

  1. 458030 河南省鹤壁市人民医院检验科（李金丽）, 鹤壁职业技术学院（宋晓光）
  • 收稿日期:2017-09-11 出版日期:2018-02-20 发布日期:2018-02-28
  • 作者简介:李金丽（1972-）,女,学士,主要从事医学检验免疫、微生物工作,（Tel）15139260138。

Comparison of Group B Streptococcus Two Detection Methods

LI Jin-li, SONG Xiao-guang   

  1. Hebi City People's Hospital of Henan Hebi 458030
  • Received:2017-09-11 Online:2018-02-20 Published:2018-02-28

PDF

1504

摘要： 目的 选择一种快速检测GBS菌的实验方法,并对该方法进行评价。方法 以细菌培养法为标准方法,对752例阴道棉拭子进行培养鉴定,同时以双抗体夹心法为原理的B群链球菌免疫层析法为实验方法,分析免疫层析法的灵敏度、特异性、Kappa值,根据Kappa值比较两种方法的一致性。结果 免疫层析法的灵敏度为98.21%,特异性为93.75%,诊断符合率94.4%,Kappa=0.807。结论 免疫层析法对GBS检测有较高的灵敏度和特异性,诊断符合率较高,且操作简便,适合基层医疗机构开展GBS菌的筛查。

关键词: B群链球菌, 免疫层析法, 细菌培养

Abstract: Objective A method for rapid detection of GBS bacteria is chosen,then it is evaluated. Methods The bacterial culture as the standard method,752 cases of vaginal swabs were cultured and identified,while the double antibody sandwich method for group B streptococcus immune chromatography was tested,Kappa value from SPSS19.0 software analysis of sensitivity and specificity of immunochromatography was used to compare the two Methods of consistency in the detection. Results The sensitivity of the immunochromatography was 98.21%,a specificity of 93.75%,diagnosis rate of 94.4%,Kappa=0.807. Conclusion The immune chromatography detection of GBS has high sensitivity,specificity,and high compliance rate with standard method in the detection of group B streptococcus,and is easy to operate,suitable for primary health care institutions GBS bacteria screening.

Key words: B streptococcus, Immunochromatography, Bacterial culture

中图分类号: