• 中国科学论文统计源期刊
  • 中国科技核心期刊
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临床输血与检验

临床输血与检验 ›› 2018, Vol. 20 ›› Issue (6): 650-652.DOI: 10.3969/j.issn.1671-2587.2018.06.031

• 血型与临床 • 上一篇    下一篇

两种血型分析系统在直接抗人球蛋白实验中的应用比较

刘自强, 杨光, 孙进   

  1. 610000 四川省医学科学院,四川省人民医院
  • 收稿日期:2018-05-09 出版日期:2018-12-20 发布日期:2018-11-12
  • 作者简介:刘自强（1985-）,男,四川武胜人,检验师,学士,主要从事检验输血工作,（Tel）18111624698。

Comparison of the Performance of the 2 Types of Automatic Blood Grouping Instruments in Direct Antiglobulin Test

LIU Zi-qiang, YANG Guang, SUN Jin   

  1. Sichuan Academy of Medical sciences,SichuanProvincial People's Haspital 610000
  • Received:2018-05-09 Online:2018-12-20 Published:2018-11-12

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摘要： 目的 比较2种全自动血型分析系统在直接抗人球蛋白实验（DAT）中的准确性和敏感性。方法 随机选择500例患者标本,用强生ORTHO AutoVue Innova全自动血型分析系统和爱康Aigel300与江阴力博卡组成的血型系统分别进行直接抗人球蛋白实验,最后用试管法抗人球蛋白实验进行验证,分析比较2台血型系统的假阳性率﹑假阴性率和仪器性能。结果 强生ORTHO AutoVue Innova全自动血型分析系统假阳性率为8.98%,未发现假阴性标本。爱康Aigel300与江阴力博卡血型系统,假阴性率为30.6%,假阳性率2.25%,经分析2台仪器检查结果的差异有统计学意义（P<0.05）。结论 2台全自动血型系统在直接抗人球蛋白实验中,强生ORTHO AutoVue Innova全自动血型仪具有明显的性能优势和临床应用价值。

关键词: 血型仪, 直接抗人球蛋白实验

Abstract: Objective To observe the accuracy and sensitivity of the 2 types of Automatic Blood Grouping Instruments in Direct Antiglobulin Test （DAT）,we compare the false-positive rate,false-negative rate as well as the performances of instruments between the Automation Blood Grouping Analyser System of ORTHO AutoVue Innova and the blood grouping system which were composed of Ai gel 300 and the antigen detection card of Jiangyin Libio. Methods 500 randomLy selected patient samples were detected for DAT by above two types of instruments. The results were verified by Intube method of DAT. Results The ORTHO AutoVue Innova got the false-positive rate of 8.98% without detecting any false-negative sample. Meanwhile,the blood grouping system composed of Ai gel 300 and the antigen detection card of Jiangyin Libio showed 30.6% of the false-negative rate of and 2.25% of the false-positive rate. There was significant difference in detection between these two systems（P<0.05）. Conclusion The results show that ORTHO AutoVue Innova has obvious better performances and more clinical practice value in DAT.

Key words: Blood grouping instruments, Direct antiglobulin test （DAT）

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