• 中国科学论文统计源期刊
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临床输血与检验

临床输血与检验 ›› 2019, Vol. 21 ›› Issue (1): 67-69.DOI: 10.3969/j.issn.1671-2587.2019.01.023

• 临床检验 • 上一篇    下一篇

运输温度变化对酶免试剂检测结果的影响

师玉红, 王新梅, 姜燕娟, 聂军, 郭欣   

  1. 453000 河南省新乡市中心血站
  • 收稿日期:2018-06-21 出版日期:2019-02-20 发布日期:2019-01-28
  • 作者简介:师玉红（1969-）,女,河南新乡人,副主任技师,学士,主要从事临床输血工作,（Tel）13937369111。

Effect of Transportation Temperature of Reagents on the Test Results in Enzymatic Immunoassays

SHI Yu-hong, WANG Xin-mei, JIANG Yan-juan, et al   

  1. Xinxiang Central Blood Station,Henan,453000
  • Received:2018-06-21 Online:2019-02-20 Published:2019-01-28

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摘要： 目的 探讨运输温度变化对酶免试剂检测结果的影响。方法 对购进的酶免试剂进货检验的同时,对同批次试剂模拟不同运输温度下的运输过程,检测试剂在受到外界环境温度变化下引起的质量变化。结果 在37℃±1℃、45℃±1℃运输酶免试剂时,实验2组、实验3组在第5天、第7天的结果与对照组比较,差异有统计学意义（P<0.05）。结论 当酶免试剂在高温环境下运输时,酶免试剂的检测能力下降,易漏检弱阳性标本。

关键词: 温度, 酶免试剂

Abstract: Objective To investigate the effect of transportation temperature of the reagent on the quality and reliability of the results of enzymatic immunoassay. Method The purchased reagents were subjected to various temperatures simulated to the process of outside transportation. The quality of the reagents and reliability of tests were assessed. Results The quality of the reagents was remarkably decreased when they were exposed to 45℃±1℃ compared to their exposure to 37℃±1℃（P<0.05）. Conclusion Exposure of the reagents to high temperature during transportation may attenuate the quality of the reagents and results of laboratory tests,leading to missing of some weakly positive samples.

Key words: Temperature, Enzyme immunoassay reagents

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