Abstract: Objective To establish normal reference range of LHD（low hemoglobin density） and MAF （Microcytic Anemia Factor） in the healthy crowds from Jiangning hospital,and to study clinical values in the two parameters' joint detection in the diagnosis of iron deficiency Anemia. Methods To select randomly 180 cases of healthy exam crowds,among which 90 cases were male,and the other 90 cases were female,54 cases of iron-deficiency anemia IDA and 50 patients with non iron deficiency anemia. Routine blood tests were operated by UniCe®DxH 800,and the hematology analyzer calculates the LHD and MAF,based on the analysis of parameters of MCHC （mean corpuscular hemoglobin concentration）,MCV（erythrocyte mean corpuscular volume）,and Hb（hemoglobin,Hb）.Results the average value of LHD in the healthy control was 2.11%,95% confidence interval of which was 0.65% ~ 3.79%. The LHD value of males was higher than that of females. The difference was statistically significant （t=4.45,P<0.01）. The average value of MAF was 130,95% confidence interval of which was 111.35 ~ 146.19. The MAF value of males was higher than that of females,the difference was statistically significant（t=9.14,P<0.01）. LHD in the IDA group was obviously higher,average of which was 26.19%,compared with the healthy control group,and the difference was statistically significant （t=10.93,P<0.01）;There were significant differences in the group of anemia patients with non iron deficiency anemia（t=4.68,P<0.01）.There was a significant decline in MAF,the average of which was 78.88,compared with the healthy control group,and the difference was statistically significant（t=26.10,P<0.01）.There were significant differences in the group of anemia patients with non iron deficiency anemia （t=7.6,P<0.01）. Receiveroperating characteristic（ROC） curve analysis for LHD and MAF in the diagnosis of IDA：cut-off 4.09% and 109.9.The sensitivity and specificity of diagnosis for IDA were higher than MCHC. The sensitivity and specificity of MAF were optimal. Conclusion To establish the reference rang of LHD and MAF in the area of jiangning.LHD and MAF for early diagnosis of IDA has certain clinical significance.

Key words: Low hemoglobin density, Microcytic anemia factor, Confidence interval, Iron deficiency anemia