Abstract: Objective To observe the manual and automatic separation machine and the use of ACP-215 automatic red blood cell processing system in the preparation of washed red blood cell quality control indicators，compare the three . Methods on the quality of red blood cell differences. Method 120 cases of 2 U washed red blood cell products prepared by ACP-215 erythrocyte washing machine and 30 cases of similar products prepared by hand washing and 30 cases of automatic blood component separation were prepared. Results The hemoglobin content，supernatant protein content and hemolysis rate were 48.85±6.44，0.15±0.07 and 0.16±0.05，respectively，when the quality of washed red blood cell was determined by using ACP-215 erythrocyte washing machine. The other two . Methods of preparation data are good，are in line with national standards. Conclusion The use of ACP-215 erythrocyte washing machine to prepare washed red blood cell product quality is more superior，can further ensure the safe and effective clinical infusion.

Key words: ACP-215 red blood cell washing machine, Automatic separation machine, Washing red blood cell Quality