Abstract: Objective To evaluate the effects of the populations of the Tibetan and Han Plain on the function of red blood cells carrying oxygen in different storage conditions. Method sAnticoagulation was collected from the populations of 7 the Tibetan and 6 Han Plain and red blood cells were saved with different preservation solutions (4℃ EDTA anticoagulant,4℃ red blood cell preservation solution,compound glycerol solution -80℃). After three days red blood cell P₅₀ was detected under the above conditions. The pH of the 4℃ EDTA anticoagulant was also detected for analyzing the reasons that may affect P₅₀. Results The P₅₀ datas of all specimens of Tibetan and Han nationality were lower than those of normal people. The P₅₀ data in the preservation solution at 4℃ was higher than those in the 4℃ EDTA anticoagulant and whole blood of the frozen solution which was stored in glycerol solution -80℃. The P₅₀ data of the red cell of the Tibetan stored in the preservation solution at 4℃ was higher than that of Han Plain. At the same time,there is no significantly difference in the P₅₀ data of whole blood which was stored at 4℃ or-80℃ ConclusionDifferent preservation solutions could have significant effect on P₅₀ data of red blood cells. There is no difference between the three different preservation solutions on the P₅₀ of Tibetan an Han population. The P₅₀ of red blood cells stored in the CAPD-1 preservation sloution of the two groups were relatively high,and the preservation solution had little effect on the red blood cell carrying oxygen. Therefore the above conclusions will provide guiding significance for blood preservation and transfusion of patients,especially for high altitude blood transfusion.

Key words: Red blood cell presevation, Oxygen carrying capacity, P₅₀