Abstract: Objective To explore the difference of the red blood cells (RBCs) in Raman spectrum and P₅₀ values in people living in the plain and the plateau for various durations and to analyse the effect of hypoxia on the structure of human hemoglobin. Method Thirty healthy males were selected,with 15 in each. The peripheral anticoagulant blood samples were collected and the hemoglobin was used for detection by Raman spectrum and suspension of the RBCs was subjected to the P₅₀ value determination. Results Compared with those residing in the plain,distinct peaks of Raman spectrum were noted on the locations of 1 341 cm^-1,1 375cm^-1,1 585 cm^-1,and 1 638 cm^-1,respectively,after 3 and 7 days in the men of rush entrance into the plateau. After 30 days of acclimatization training on the plateau,the characteristic peaks of the spectrum disappeared. The P₅₀ value increased with the rapid entry to the plateau from 3 to 7 days whereas decreased following acclimatization for 30 days. Conclusion The hemoglobin structure modifications exist in those who have a rush advance to the highland,with the P₅₀ value increasing.

Key words: Raman, spectroscopy P₅₀ value, Plateau acclimatization