Abstract: Objective Analyze the influencing factors on Rh（CcEe）alloimmunization in patients with liver cirrhosis after ABO,Rh（D）isotype random matched blood transfusion. Method A total of 301 liver cirrhosis patients who received blood transfusion in the hepatobiliary regions of our hospital from January 1,2019 to June 30,2021 were included,including 206 males and 95 females. Patients with incomplete clinical data and Rh（CcEe） alloantibody detected in the first admission were excluded. Univariate and multivariate Logistic regression were carried out to analyze the influencing factors on Rh（CcEe）alloimmunization in patients with liver cirrhosis after ABO, Rh（D） isotype random matched blood transfusion. Result （1）Within the 301 patients,the positive rate of Rh（CcEe）alloantibody was 6.6%（20 / 301）. The percentage of anti-E antibody, anti-Ec composite antibody,anti-Ec mixed antibody,anti-C antibody,anti-Ce composite antibody were 70.0% （14 /20）,5.0%（1 / 20）,10.0%（2 / 20）,10.0%（2 / 20）,5.0% （1 / 20）,respectively.（2）There were statistical differences in the alloimmunization of Rh（CcEe）in patients with liver cirrhosis after ABO, Rh（D）isotype random matched blood transfusion with different causes,different red blood cell transfusion times and different blood types（P<0.05）.（3）Univariate Logistic regression analysis showed that liver cirrhosis after autoimmune hepatitis, red blood cell transfusion 5~9 times,red blood cell transfusion times more than or equal to 10 and type O blood were the risk factors of the Rh（CcEe）alloimmunization in patients with liver cirrhosis. The OR values were 6.888（95%CI：2.022~23.461）、14.444（95%CI：2.980~70.024）、30.469（95%CI：6.380~145.508）、12.444（95%CI：1.597~97.001） respectively. （4）Multivariate Logistic regression analysis indicated that liver cirrhosis after autoimmune hepatitis, red blood cell transfusion 5~9 times, red blood cell transfusion times greater than or equal to 10 and type O blood were the risk factors of the Rh（CcEe）alloimmunization in patients with liver cirrhosis. The OR values were16.909（95%CI：2.402~119.021）、22.311（95%CI：3.496~142.387）、36.527（95%CI：5.758~231.718）、10.160（95%CI：1.061~97.281）respectively.Conclusion The risk of Rh（CcEe） alloantibody is higher in patients with liver cirrhosis after ABO,Rh（D） isotype random matched blood transfusion.Liver cirrhosis after autoimmune hepatitis,red blood cell transfusion times,and type O blood were the risk factors of the Rh（CcEe）alloimmunization in patients with liver cirrhosis,especially those with 5~9 times of blood transfusion and greater than or equal to 10 times of red blood cell transfusion.It is recommended to adopt ABO,Rh（DCcEe）isotype blood transfusion for patients with liver cirrhosis.

Key words: Liver cirrhosis, Randomized matched blood transfusion, Alloimmunization, Cross-sectional study