Abstract: Objective To study the serological characteristics, the strategy of blood transfusion and the molecular method of chimera. Methods The serology test of ABO and Rh blood group were done to confirm the four patients ABO and RhD blood group from hospital. Separation of newborn red blood cells by capillary centrifugation, absorb test, DTT treatment of agglutination erythrocyte test and molecular biological identification for one case. Results the patient 1, the blood group of ABO is AB/B chimeras, the blood group of Rh is the gene of RhCE chimeras; the patient 2, the blood group of Rh is the gene of RhD chimeras; the patient 3, the blood group of ABO is AB/B chimeras, the blood group of Rh is the gene of RhD chimeras; the patient 4, the blood group of ABO is O/B chimeras, the blood group of Rh is the gene of RhCE chimeras. Conclusions In terms of blood group serology, the main characteristic is mixed appearance on ABO and Rh blood groups in the absence of a recent history of transfusion and a disease that weaken the patient's antigens. Based on this, if there is a suspicious blood group chimera it can be confirmed by serological and molecular biological methods.

Key words: Blood group chimera, ABO chimera, Rh chimera, Capillary centrifugation, Melting curve