Abstract: Objective To investigate the accuracy, sensitivity and specificity of the microfluidic gel method in ABO blood group normalisation and Rh blood group antigen detection. Methods Samples from 1 005 patients with clinical blood use were tested for positive ABO typing and Rh blood grouping by microfluidic gel technology versus column agglutination technology,and the positive, negative and total compliance rates of the two methods were compared, as well as the compliance situation in patients with different diseases and ages. Results Compared with the column agglutination method, the compliance rate of microfluidic gel technology in ABO positive typing and Rh blood typing was 99.70% and 98.61%, respectively. There were 17 cases of incompatible identification results, including 5 cases of haematological diseases, 3 cases of tumours, 4 cases of infectious diseases and 5 cases of other diseases; including 6 cases of history of blood transfusion and 7 cases of history of pregnancy and childbirth; there was no significant difference between the results of the two detection methods with statistical analysis (P>0.05, Kappa value>0.75). Conclusion The microfluidic gel method has the advantages of low sample and reagent consumption, small test card size and convenient operation, and is expected to be popularised in clinical testing due to its high sensitivity, good specificity and accurate results in detecting ABO positive blood group typing and Rh blood group antigen.

Key words: Microfluidic gel technology, Column agglutination technology, Blood typing, ABO, Rh