Abstract: Objective To discuss the feasibility of the application of capillary centrifugation technology in RhD typing in patients with mixed field reactivity. Methods The red blood cells (RBCs) of 11 patients with mixed field reactivity in RhD typing were washed for hematocrit cells. The blood cells were separated by capillary centrifugation technology and the D antigen was identified by adding anti-D reagents into the proximal and distal red blood cells. The D antigen of transfused RBCs was traced against the blood donor code. Results By capillary centrifugation, RBCs showed 1 RhD- negative, 8 RhD-positives and 2 mixed field reactivities. Conclusion Capillary centrifugation technology can effectively separate the new RBCs to identify the D antigen to provide blood transfusion safety．

Key words: Capillary centrifugation, RhD antigen, Blood type identification