Analysis of the Effect of RhCcEe Phenotype-matched Transfusion in Patients with RhCcEe Mixed-field Phenotype Identified by Capillary Ultracentrifugation

doi:10.3969/j.issn.1671-2587.2025.06.008

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2025, Vol. 27 ›› Issue (6): 796-801.DOI: 10.3969/j.issn.1671-2587.2025.06.008

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Analysis of the Effect of RhCcEe Phenotype-matched Transfusion in Patients with RhCcEe Mixed-field Phenotype Identified by Capillary Ultracentrifugation

SONG Zhiqun, LI Ying, ZHU Wenwen, SUN Xiaohong, ZHOU Xiaoyu

Department of Blood Transfusion, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029

Received:2025-04-14 Published:2025-12-24

Abstract

Abstract: Objective To explore the value of capillary ultracentrifugation for RhCcEe phenotype identification in patients with mixedfield agglutination reaction (MF). Methods A total of 1 155 patients with MF in RhCcEe phenotype identification from December 2020 to April 2024 were enrolled at First Affiliated Hospital of Nanjing Medical University. The young erythrocytes were separated by capillary ultracentrifugation and identified the RhCcEe antigen, then retrospectively analyzed the RhCcEe antigen compatibility of recipients and donors. Results The distribution of Rh blood groups in 995 (86.15%) patients successfully identified by capillary ultracentrifugation was CCDee>CcDEe>ccDEE>CcDee. The proportion of RhCcEe antigen incompatibility transfusion was high (35.41%). When recipients received blood transfusions from multiple donors, their risk of Rh antigen incompatibility transfusion reactions was significantly increased (P<0.001). Conclusion Capillary ultracentrifugation was helpful for identifying Rh blood groups in patients with MF of RhCcEe antigens and provided the basis for compatible blood transfusion

Key words: Capillary ultracentrifugation, Rh blood group identification, Compatible blood transfusion

CLC Number:

R457.1

SONG Zhiqun, LI Ying, ZHU Wenwen, SUN Xiaohong, ZHOU Xiaoyu. Analysis of the Effect of RhCcEe Phenotype-matched Transfusion in Patients with RhCcEe Mixed-field Phenotype Identified by Capillary Ultracentrifugation[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(6): 796-801.

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Analysis of the Effect of RhCcEe Phenotype-matched Transfusion in Patients with RhCcEe Mixed-field Phenotype Identified by Capillary Ultracentrifugation

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[1]	ZHANG Yuan-qin, WANG Mei-ru, XU Lu, et al. Study of Capillary Ultracentrifugation Technique on Prediction of Massive Maternal Transfusion Syndrome in Vitro [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(3): 301-305.
[2]	LIU Li-na, YIN Wen, HU Xing-bin. Application of Capillary Ultracentrifugation in Identification of Rh Blood Group in the Patients after Transfusion [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2018, 20(6): 643-646.