A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications

doi:10.3969/j.issn.1671-2587.2026.03.020

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2026, Vol. 28 ›› Issue (3): 425-429.DOI: 10.3969/j.issn.1671-2587.2026.03.020

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A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications

KANG Shirong, WANG Dan, YUAN Xiaohua, LU Hongqin, LI Suliang

The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710000

Received:2025-10-28 Published:2026-07-07

Abstract

Abstract: Objective To identify the specificity, immunoglobulin subclass and titer of an anti-K antibody detected in a patient with a positive irregular antibody screen, and to analyze potential etiological factors contributing to its formation. Methods ABO blood grouping and Rh phenotyping were performed using standard serological methods. Irregular antibody screening and identification were carried out by normal saline (NS) tube test and microcolumn gel test (MGT). Crossmatching was conducted using NS, MGT, and manual polybrene test (MPT). The serum was treated with 2-mercaptoethanol (2-ME) to determine the immunoglobulin class and titer of the antibody. Results The irregular antibody screen was positive, and the antibody was specifically identified as anti-K. It was characterized as a mixed IgM and IgG isotypes, with an IgM titer of 2 and an IgG titer of 256. Conclusion Utilization of suitable laboratory techniques allows for the accurate detection of IgM+IgG anti-K antibodies, providing a guarantee for safe clinical transfusion.

Key words: Kell blood group, Anti-K antibody, Antibody identification, IgM antibody, IgG antibody

CLC Number:

R457.1⁺1

KANG Shirong, WANG Dan, YUAN Xiaohua, LU Hongqin, LI Suliang. A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 425-429.

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A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications

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