Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing

doi:10.3969/j.issn.1671-2587.2026.03.011

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2026, Vol. 28 ›› Issue (3): 363-368.DOI: 10.3969/j.issn.1671-2587.2026.03.011

Previous Articles Next Articles

Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing

XU Zhiyuan, LI Xiaofei, LIU Tingting, ZHENG Zhongnan, MIAO Tianhong

Beijing Red Cross Blood Center, Beijing 100088

Received:2026-03-18 Published:2026-07-07

Abstract

Abstract: Objective To investigate the influence of reagent-dependent antibodies on blood transfusion compatibility testing and to explore potential resolution strategies. Methods Five difficult suspected reagent-dependent antibody generated patient samples referred by clinical hospitals were analyzed. ABO blood grouping, antibody screening, antibody identification and crossmatching were performed using the in vitro anti-human globulin method and the anti-human globulin card method. Results Sample 1 had antibodies against the red blood cell suspension medium, Sample 2 exhibited reagent antibodies against the anti-typing cell suspension medium, Sample 3 displayed reagent antibodies against the enhancer medium Liss, and Sample 4 also had reagent antibodies against the enhancer medium Liss, both showing similar anti-C and anti-e specificityies. Samples 5 had antibodies against the albus reagent. Conclusion By conducting a comprehensive analysis and comparing the different results obtained from different reagents, reaction media, and detection methods for the same patient sample, the specific reagent-denpendent antibody can be accurately identified.

Key words: Reagent-dependent antibody, Antibody screening, Antibody identification, Blood transfusion safety

CLC Number:

R457.1

XU Zhiyuan, LI Xiaofei, LIU Tingting, ZHENG Zhongnan, MIAO Tianhong. Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 363-368.

References

[1] 中华人民共和国国家卫生健康委员会. 国家卫生健康委办公厅关于印发临床用血技术规范(2025年)的通知[EB/OL]. (2025-12-04). https://www.nhc.gov.cn/ylyjs/zcwj/202512/1f0e99982b49476b9b076ee10ef9ca75.shtml.
[2] 中华人民共和国国家卫生健康委员会. 输血相容性检测标准[S]. 2022.
[3] Chapman JF, Elliott C, Knowles SM, et al.Guidelines for compatibility procedures in blood transfusion laboratories[J]. Transfus Med. 2004,14(1):59-73.
[4] Claudia s.Cohn,Meghan Delaney,Susan T.Johnson,et al. AABB technical manual[M]. 20th edition,2020:292-293.
[5] Patch G C,Hutchinson C F,Lang N A,et al.How to recognize and resolve reagent-dependent reactivity:a review[J]. Immunohematology,2016,32(3):96-99.
[6] Tobar M.A case study:an ABO discrepancy due to an antibody to EDTA[J]. Immunohematology,1987,3(3):40-41.
[7] Rajendran A,Krishna G,Suresh B,et al.Red cell incompatibility due to antibody against ingredient in column matrix:A rare entity[J]. J Clin Sci Res,2016, 5(1):40.
[8] Howard P L.Blood bank reagents:some problems related to preservatives and dyes[J]. Transfusion,1976, 16(2):166-169.
[9] Anuragaa S,Sahoo D,Abhishekh B,et al.A rare case of antibody against enhancement media interfering with crossmatching:A case report and review of literature[J]. Asian J Transfus Sci,2021,15(1):100-103.
[10] Pham B N,Gien D,Bensaad F,et al.Antibodies to co-trimoxazole (trimethoprim and/or sulfamethoxazole) related to the presence of the drug in a commercial low-ionic-strength solution[J]. Transfusion,2012,52(4):844-848.
[11] Mason J M,Osborne P T,Hall A J,et al.Example of a thimerosal-dependent antibody without apparent blood group specificity[J]. Vox Sang,1985,48(5):313-316.
[12] Judd W J,Steiner E A,Cochran R K.Paraben-associated autoanti-Jk^a antibodies:Three examples detected using commercially prepared low-ionic strength saline containing parabens[J]. Transfusion,1982,22(1):31-35.
[13] Garratty G.In vitro reactions with red blood cells that are not due to blood group antibodies:a review[J].Immunohematology,1998,14(1):1-11.
[14] Reviron M,Janvier D,Reviron J,et al.An anti-I cold auto-agglutinin enhanced in the presence of sodium azide[J]. Vox Sang,1984,46(4):211-216.
[15] Reid M E,Ellisor S S,Frank B A.Another potential source of error in Rh-hr typing[J]. Transfusion,1975, 15(5):485-488.
[16] Beattie K M,Seymour D S,Scott A.Two further examples of acriflavin antibody causing ABO cell typing errors[J]. Transfusion,1971,11(2):107-109.

Impacts of Reagents-dependent Antibodies on Blood Transfusion Compatibility Testing

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

About

Author

Journal

Access Statistics

Contact

[1]	KANG Shirong, WANG Dan, YUAN Xiaohua, LU Hongqin, LI Suliang. A Case of Mixed IgM and IgG Anti-K Antibody: Identification and Clinical Implications [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(3): 425-429.
[2]	MA Jingsi, ZHAO Ning, DENG Jiang, ZHANG Yanyu. Progress in Detection Technologies for Bacterial Pathogens in Platelets [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(5): 667-673.
[3]	XU Haiyan, ZHANG Yining, LIANG Yan, XING Yan, XU Yali, CUI Ying. Retrospective Analysis of the Distribution of ABO Blood Types, Rh Phenotypes, and Irregular Antibodies in 7 567 Hospitalized Patients with Malignant Tumors [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(4): 462-469.
[4]	WANG Yilai, HE Ziyi. Dengue Fever and Blood Transfusion Safety [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(3): 427-432.
[5]	MA Chunya, ZHANG Min, FU Lihui, ZHU Jianling, LUO Yuanyuan, GUAN Xiaozhen, YU Yang. Laboratory-based Diagnostic Investigation of Drug-induced Thrombocytopenia from Piperacillin-tazobactam Sodium [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(2): 191-195.
[6]	YI Meng, FAN Bin, DUAN Xiaoqiong, CHEN Limin. Influence of Occult Hepatitis B Infection on Blood Transfusion Safety and Countermeasures [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2025, 27(2): 254-260.
[7]	ZHENG Zhongnan, LI Xiaofei, ZHANG Ye, MIAO Tianhong. Multi-blood Group System Antibody Identification and Cross-matching Strategies: One Case of Anti-f Combined with Anti-M [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(1): 132-135.
[8]	LI Zhengfeng, WANG Jia, CHEN Ping, et al. A Cross-sectional Study on the Distribution and Influencing Factors of Unexpected Antibody Positive Results in Pediatric Hospitalized Patients Planning to Receive Blood Transfusion [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2023, 25(3): 374-378.
[9]	XIE Hui-yi, ZHOU Zai-xin, YANG Yue, et al. Analysis of the Influence of Anti-M Antibody on Blood Group Identification and Clinical Blood Transfusion [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(6): 716-720.
[10]	HE Xi-rong, ZHANG Fan, WANG Na, et al. 0.01 mol/L DTT Treatment of Accidental Antibody Screening Reagent Erythrocytes to Eliminate Daratumumab Interference and its Clinical Adaptability [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2022, 24(6): 749-753.
[11]	WANG Wen-ting, LIU Qi-wang, ZHANG Rong, et al. Analysis and Discussion on Clinical Blood Transfusion and its Curative Effect in Patients with Parent Uterus Transplantation [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(5): 551-555.
[12]	YAN Ye, ZHANG Qin, WANG Li-na, et al. Combined Application of Screening Cells with Two Different Antigen Patterns [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2021, 23(3): 324-327.
[13]	LI Wei-Chao, CHENG Dao-Sheng. The Blood Matching Strategy of CD38 Monoclonal Antibody in Treating Multiple coli Myeloma [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(6): 624-626.
[14]	WANG Li, LIU Ying, LI Wen-juan, et al. Screening Results and Risk Factors Analysis of Irregular Antibodies in 8 775 Patients Receiving Blood Transfusions [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(5): 480-483.
[15]	CAI Juan, SUN Hong. Analysis of Weak Positive Results of Irregular Antibody Screening [J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2020, 22(5): 484-486.