Abstract: Objective To explore the significance of combined application of screening cells with different antigen patterns to avoid missed detection of irregular antibodies. Methods Plasma specimens of 1 200 patients undergoing blood transfusion were selected. Gel test was used for irregular antibodies. Positive specimen was further evaluated for identification of specific alloantibody with cell panel. Negative specimen was tested by different methods and screening cells. Results The total positive rate of irregular antibodies was 0.75% (9/1 200), of which the positive rate tested by a single reagent was 0.58% (7/1 200). Two different antigen patterns were used to screen the remaining negative samples, two samples were positive and the missed detection rate was 0.17% (2 /1 193). Conclusion The use of papain enzymatic method can increase the positive rate of alloantibodies detection. The combined application of screening cells with different antigen patterns can effectively prevent the missed detection of irregular antibodies and reduce the occurrence of transfusion reactions.

Key words: Irregular antibody screening, Antibodies identification, Papain