Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment

doi:10.3969/j.issn.1671-2587.2026.04.006

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2026, Vol. 28 ›› Issue (4): 496-503.DOI: 10.3969/j.issn.1671-2587.2026.04.006

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Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment

SU Xiaomin, ZHANG Guoquan, WANG Yaqiong, XU Hua

Shaanxi Blood Center, Xi'an 710061

Received:2026-07-08 Online:2026-08-20 Published:2026-08-26

Abstract

Abstract: Objective This study aimed to determine the quality indicators, proteomic and metabolomic profiles of washed red blood cells (WRBCs), and further evaluate their quality alterations at the end of storage via correlation analysis. Methods Suspended RBCs were categorized into two groups: the control group (Control5w), which did not undergo washing treatment, and the saline-washed group (Saline5w). Following storage at 4 ℃, samples were collected at the end of storage, and relevant quality control indicators for suspended RBCs were examined. Proteomic and metabolomic analyses were performed to assess changes in biological functions and correlations during the storage process of the two groups using bioinformatics analysis. Results After 35 days of storage at 4 ℃ in the saline group, all RBC indicators complied with national standards for the quality of WRBCs. The comprehensive integration and analysis of proteomics and metabolomics data have elucidated alterations in the protein and metabolite composition of RBCs washed with saline. This was achieved by examining the correlations among various biomolecular levels. Conclusion This study substantiates the investigation of the physiological and biochemical functions of washed RBCs through the application of advanced proteomic and metabolomic methodologies.

Key words: Washed red blood cells, Proteomics, Metabolomics, Integrated multi-omics analysis, Quality evaluation

CLC Number:

Q493.2

SU Xiaomin, ZHANG Guoquan, WANG Yaqiong, XU Hua. Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2026, 28(4): 496-503.

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Proteomic and Metabolomic Analyses of Washed Red Blood Cells with Key Quality Assessment

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