The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

doi:10.3969/j.issn.1671-2587.2024.03.003

JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE ›› 2024, Vol. 26 ›› Issue (3): 308-314.DOI: 10.3969/j.issn.1671-2587.2024.03.003

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The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

MENG Weicheng, CHEN Yaozhen, WANG Yafen, AN Ning, LIU Zhixin, WU Xiaoshuang, WANG Fei, HU Xingbin

Department of Blood Transfusion, Xijing Hospital, Air Force Military Medical University, Xi'an 710032

Received:2024-02-26 Online:2024-06-20 Published:2024-06-24

Abstract

Abstract: Objective To explore the underlying causes for the observed anomalies, the oxygen-carrying/releasing capacity of sensitized red blood cells (RBCs) was analyzed . Methods The sensitized red blood cells were detected through microcolumn gel cards, and an oxygen-carrying/releasing assessment device was employed to evaluate the oxygen-carrying/releasing capacity of sensitized RBCs in patients with autoimmune hemolytic anemia (AIHA) and hemolytic disease of the newborn (HDN). We constructed a transfusion-induced hemolytic reaction mouse model using plasma from healthy adults, while the control group received PBS. Record the time until exhaustion during weight-loaded swimming in mice and analyze the oxygen-carrying/releasing capacity of isolated mouse RBCs. Perform GO and KEGG enrichment analysis on differentially expressed proteins related to hemolysis and the control group using proteomics. Measure BPGM enzyme activity and 2,3-DPG levels in sensitized RBCs using ELISA. Results Compared to the corresponding control groups, the oxygen dissociation curves in AIHA, HDN, and transfusion-induced hemolytic reaction model mice exhibited a left shift and decreased P50. The weight-loaded swimming time in hemolytic model mice was significantly shortened. Proteomic screening revealed abnormalities in BPGM, with decreased BPGM enzyme activity and a decline in intracellular 2,3-DPG content. Conclusion The reduced activity of BPGM in sensitized RBCs led to a decline in their oxygen-releasing capacity.

Key words: Red blood cells, Hemolysis, BPGM, 2,3-DPG

CLC Number:

R457

MENG Weicheng, CHEN Yaozhen, WANG Yafen, AN Ning, LIU Zhixin, WU Xiaoshuang, WANG Fei, HU Xingbin. The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity[J]. JOURNAL OF CLINICAL TRANSFUSION AND LABORATORY MEDICINE, 2024, 26(3): 308-314.

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The Impact of Reduced Bisphosphoglycerate Mutase（BPGM）Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity

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